Search results for Purified anti-Neurofilament H
Purified anti-Neurofilament H (NF-H), Phosphorylated - SMI 34, BioLegend, 835504
SMI 34 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, as well as in chicken. Immunohistochemically, SMI 34 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 34. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 34 may be found also in neuronal cell bodies. In Alzheimer's disease, SMI 34 reacts with intraneuronal tangles but does not react with extraneuronal (ghost) tangles. It is the only anti-phosphoneurofilament that reacts regularly with Alzheimer tangles. It does not cross-react at concentrations of 1:20,000 with Alzheimer MAP-Tau in immunoblots, demonstrating, thereby, the presence of neurofilaments in Alzheimer tangles.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB -Verified
Conjugation
Purified anti-Neurofilament H (NF-H), Phosphorylated - SMI 34, BioLegend, 835503
SMI 34 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, as well as in chicken. Immunohistochemically, SMI 34 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 34. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 34 may be found also in neuronal cell bodies. In Alzheimer's disease, SMI 34 reacts with intraneuronal tangles but does not react with extraneuronal (ghost) tangles. It is the only anti-phosphoneurofilament that reacts regularly with Alzheimer tangles. It does not cross-react at concentrations of 1:20,000 with Alzheimer MAP-Tau in immunoblots, demonstrating, thereby, the presence of neurofilaments in Alzheimer tangles.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB -Verified
Conjugation
Purified anti-Neurofilament H (NF-H), Phosphorylated Antibody - SMI 31, BioLegend, 801602
Additional reported applications (for the relevant formats) include: Western blotting1, immunohistochemistry2,4, and immunocytochemistry4.SMI 31 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, which chicken and frog (Xenopus). Immunocytochemically, SMI 31 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 31. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 31 may be found also in neuronal cell bodies. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures with SMI 31 by agents that induce stress-activated protein kinase. In its reaction with paired helical filaments in hereditary inclusion body myopathy, SMI 31 colocalizes with nitric oxide synthase, suggesting that oxidative stress may play a role in the pathogenic cascade of such degenerative diseases. SMI 31 co-immunoprecipitates neurofilament-associated kinase (NAK 115) via reaction of the antibody with the tail domain of neurofilament H.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB, ICC -Verified
Conjugation
Purified anti-Neurofilament H (NF-H), Phosphorylated Antibody - SMI 31, BioLegend, 801601
Additional reported applications (for the relevant formats) include: Western blotting1, immunohistochemistry2,4, and immunocytochemistry4.SMI 31 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, which chicken and frog (Xenopus). Immunocytochemically, SMI 31 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 31. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 31 may be found also in neuronal cell bodies. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures with SMI 31 by agents that induce stress-activated protein kinase. In its reaction with paired helical filaments in hereditary inclusion body myopathy, SMI 31 colocalizes with nitric oxide synthase, suggesting that oxidative stress may play a role in the pathogenic cascade of such degenerative diseases. SMI 31 co-immunoprecipitates neurofilament-associated kinase (NAK 115) via reaction of the antibody with the tail domain of neurofilament H.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB, ICC -Verified
Conjugation
Purified anti-Neurofilament H NF-H Antibody anti-Neurofilament H (NF-H) - Poly28226, BioLegend, 822601
This antibody is effective in immunoblotting (WB) and immunofluorescence (IF). This antibody does not bind to protein A or protein G.* Predicted MW = 200-220 kD
Host
Chicken
Reactivity
Human, Rat, Mouse, Cat, Chicken
Applications
WB - Quality testedICC, IHC-F - Reported in the literature, not verified in house
Conjugation
Purified anti-Neurofilament H & M (NF-H/NF-M), Non-Phosphorylated Antibody - SMI 33, BioLegend, 835403
Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons.
Host
Mouse
Reactivity
Rat, Mouse, Human
Applications
IHC-P -Quality testedWB -Verified
Conjugation
Purified anti-Neurofilament H & M (NF-H/NF-M), Non-Phosphorylated Antibody - SMI 33, BioLegend, 835404
Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons.
Host
Mouse
Reactivity
Rat, Mouse, Human
Applications
IHC-P -Quality testedWB -Verified
Conjugation
Purified anti-Neurofilament H NF-H Antibody anti-Neurofilament H (NF-H) - SMI 37, BioLegend, 835803
SMI 37 reacts with a non-phosphorylated epitope in neurofilament H (NF-H) of most mammalian species. Immunocytochemically, SMI 37 visualizes some neuronal cell bodies, dendrites and thick axons in the central and peripheral nervous systems. SMI 37 staining is more restricted than that seen with SMI 32 or SMI 38 and Neuronal cell bodies are intensely stained while neuronal processes staining is less prominent. This IgM antibody reacts with goat anti-mouse IgG via the light chains common to IgG and IgM.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB -Verified
Conjugation
Purified anti-Neurofilament H NF-H Antibody anti-Neurofilament H (NF-H) - SMI 37, BioLegend, 835804
SMI 37 reacts with a non-phosphorylated epitope in neurofilament H (NF-H) of most mammalian species. Immunocytochemically, SMI 37 visualizes some neuronal cell bodies, dendrites and thick axons in the central and peripheral nervous systems. SMI 37 staining is more restricted than that seen with SMI 32 or SMI 38 and Neuronal cell bodies are intensely stained while neuronal processes staining is less prominent. This IgM antibody reacts with goat anti-mouse IgG via the light chains common to IgG and IgM.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB -Verified
Conjugation
Purified anti-Neurofilament H NF-H Antibody anti-Neurofilament H (NF-H) - SMI 38, BioLegend, 835903
SMI 38 reacts with a non-phosphorylated epitope in neurofilament H (NF-H) of most mammalian species. SMI 38 visualizes neuronal cell bodies, dendrites and some thick axons in the central and peripheral nervous systems. Other cells and tissues are unreactive.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB -Verified
Conjugation
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