Search results for immunohistochemistry
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SignalStain®Phospho-ErbB Family IHC Sampler Kit#8111, Cell Signaling Technology (CST), 8111
Each antibody in the SignalStain®Phospho-ErbB Family IHC Sampler Kit detects endogenous levels of its target protein. Phospho-EGF Receptor (Tyr1068) (D7A5) Rabbit Monoclonal Antibody detects endogenous EGF receptor only when phosphorylated at Tyr1068. This antibody may cross-react weakly with other tyrosine-phosphorylated proteins. Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit Monoclonal Antibody detects endogenous levels of ErbB2 only when phosphorylated at Tyr1221/1222. The antibody does not cross react with other activated ErbB family members or other tyrosine-phosphorylated proteins. Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit Monoclonal Antibody detects endogenous levels of HER3/ErbB3 proteins only when phosphorylated at tyrosine 1289. The antibody does not cross-react with other phosphorylated receptor tyrosine kinases at significant level. EGF Receptor (D38B1) Rabbit Monoclonal Antibody detects endogenous levels of total EGF receptor protein.
Host
Reactivity
Source / PurificationMonoclonal antibody is produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr1068 of human EGFR, Tyr1221/1222 of human ErbB2, or to Tyr1289 of human HER3/ErbB3. Monoclonal antibody to EGFR is produced by immunizing animals with a synthetic GST-fusion protein corresponding to residues containing the cytoplasmic domain of human EGF receptor.
Applications
Conjugation
SignalStain®Proliferation/Apoptosis IHC Sampler Kit#8109, Cell Signaling Technology (CST), 8109
Each antibody in the SignalStain®Proliferation/Apoptosis IHC Sampler Kit detects endogenous levels of its target protein and does not cross-react with any related proteins.
Host
Reactivity
Source / PurificationMonoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein, or with a synthetic peptide corresponding to residues surrounding Cys60 of human Survivin. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3, or with a synthetic peptide surrounding amino-terminal residues adjacent to Asp175 of human caspase-3. Antibodies are purified by protein A and peptide affinity chromatography.
Applications
Conjugation
SignalStain®Akt Pathway IHC Sampler Kit#8107, Cell Signaling Technology (CST), 8107
Each antibody in the SignalStain®Akt Pathway IHC Sampler Kit detects endogenous levels of its target protein and does not cross-react with related proteins.
Host
Reactivity
Source / PurificationMonoclonal antibody is produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding the phosphorylation site(s) of interest, and synthetic peptides corresponding to the carboxy-terminal sequence of mouse Akt or from the carboxy-terminal sequence of human PTEN.
Applications
Conjugation
Anti-KDEL | endoplasmic reticulum retention signal (50 µg) antibodies, Agrisera, AS13 2665
Immunogen affinity purified antibodies are present in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol,
Host
Rabbit
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, paraffin-embedded (IHC), Western blot (WB)
Conjugation
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