Search results for 387
Spark UV™ 387 anti-human IFN-γ Antibody, IFN-γ, 4S.B3, BioLegend, 502558
ELISA or ELISPOT Detection5:The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.Flow Cytometry3,4,6-8:The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include:neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.Note:For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Spark UV™ 387 anti-mouse CD90.2 (Thy1.2) Antibody, CD90.2, 53-2.1, BioLegend, 140335
Additional reported applications (for the relevant formats) include: immunohistochemical staining1of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-mouse CD90.2 (Thy1.2) Antibody, CD90.2, 53-2.1, BioLegend, 140336
Additional reported applications (for the relevant formats) include: immunohistochemical staining1of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human CD326 (Ep-CAM) Antibody, CD326, 9C4, BioLegend, 324255
Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry3, and spatial biology (IBEX)4,5.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human CD326 (Ep-CAM) Antibody, CD326, 9C4, BioLegend, 324256
Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry3, and spatial biology (IBEX)4,5.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-mouse Ly-6C Antibody, Ly-6C, HK1.4, BioLegend, 128059
Clone HK1.4 does not block the binding of clone RB6-8C58.Additional reported applications (for relevant formats of this clone) include:in vitroactivation of T cells1-3and immunohistochemistry of frozen sections4.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-mouse Ly-6C Antibody, Ly-6C, HK1.4, BioLegend, 128060
Clone HK1.4 does not block the binding of clone RB6-8C58.Additional reported applications (for relevant formats of this clone) include:in vitroactivation of T cells1-3and immunohistochemistry of frozen sections4.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human HLA-DR Antibody, HLA-DR, L243, BioLegend, 307689
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8,in vitroblocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human HLA-DR Antibody, HLA-DR, L243, BioLegend, 307690
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8,in vitroblocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-mouse/human CD11b Antibody, CD11b, M1/70, BioLegend, 101291
Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4,in vitroblocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).
Host
Rat
Reactivity
Mouse, Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
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