Search results for 421
Brilliant Violet 421 anti-human CD4 Antibody anti-CD4 - OKT4, BioLegend, 317434
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality testedICC - Verified
Conjugation
Brilliant Violet 421 anti-mouse CD3 Antibody anti-CD3 - 17A2, BioLegend, 100227
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Host
Rat
Reactivity
Mouse
Applications
FC -Quality testedICC -Verified
Conjugation
Brilliant Violet 421 anti-mouse CD3 Antibody anti-CD3 - 17A2, BioLegend, 100228
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Host
Rat
Reactivity
Mouse
Applications
FC -Quality testedICC -Verified
Conjugation
Brilliant Violet 421 anti-human FOXP3 Antibody anti-FOXP3 - 206D, BioLegend, 320123
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1and formalin-fixed paraffin-embedded sections1,8,19-20, and Western blotting1. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No.424401) offers improved staining and is highly recommended.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 421 anti-human FOXP3 Antibody anti-FOXP3 - 206D, BioLegend, 320124
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1and formalin-fixed paraffin-embedded sections1,8,19-20, and Western blotting1. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No.424401) offers improved staining and is highly recommended.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 421 anti-human ROR1 Antibody anti-ROR1 - 2A2, BioLegend, 357817
Clone 2A2 binds to the membrane distal Ig-domain of ROR1. Clone 2A2 has also been shown to exhibit cross reactivity towards mouse ROR12.
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
Brilliant Violet 421 anti-human ROR1 Antibody anti-ROR1 - 2A2, BioLegend, 357818
Clone 2A2 binds to the membrane distal Ig-domain of ROR1. Clone 2A2 has also been shown to exhibit cross reactivity towards mouse ROR12.
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
Brilliant Violet 421 anti-human CD45 Antibody anti-CD45 - 2D1, BioLegend, 368521
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
Brilliant Violet 421 anti-human CD45 Antibody anti-CD45 - 2D1, BioLegend, 368522
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
Brilliant Violet 421 anti-human CD80 Antibody anti-CD80 - 2D10, BioLegend, 305221
Additional reported applications (for the relevant formats) include:in vitroblocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
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