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FCM

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not verified in house

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Flow Cytometry (Fixed/Permeabilized): 1:20 Flow Cytometry (Live): 1:20

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WB ICC/IF IHC-P FCM

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Flow Cytometry (Fixed/Permeabilized): 1:50

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IF-P

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Immunohistochemistry (Paraffin): 1:100 - 1:400

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PG -Quality tested

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3D IHC -VerifiedSB -Community verifiedSB -Reported in the literature

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FC -Quality testedCyTOF®

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FC -Quality testedCyTOF® -VerifiedIHC-F

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Flow Cytometry (Fixed/Permeabilized): 1:160 Flow Cytometry (Live): 1:160

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ICC/IF FCM

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ICC/IF IHC-Fr FCM IP

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IF-F

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IHC-F - Quality tested

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IHC-F - Quality testedSB - Reported in the literature

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IHC-F -VerifiedIHC-P

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IHC-P FCM

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WB ICC/IF IHC-P FCM IP

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WB ICC/IF IHC-P IHC-Fr FCM IP Neutralizing /Inhibition

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Depletion

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FC - Quality testedCyTOF® - Verified

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FC - Quality testedIHC-F - Verified

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FCM IHC

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Flow Cytometry (Fixed/Permeabilized): 1:100 Flow Cytometry (Live): 1:100

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Flow Cytometry (Fixed/Permeabilized): 1:300 Flow Cytometry (Live): 1:300

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Flow Cytometry (Fixed/Permeabilized): 1:40 Flow Cytometry (Live): 1:40

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Flow Cytometry (Fixed/Permeabilized): 1:80 Flow Cytometry (Live): 1:80

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Flow Cytometry (Live): 1:20

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Flow Cytometry (Live): 1:80

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Functional Assays

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ICC/IF FCM IP

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ICC/IF IHC-Fr FCM IP IHC

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ICC/IF IHC-P IHC-Fr FCM

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ICC/IF IHC-P IHC-Fr FCM IP Functional Assay

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IF-Tissue

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IHC-F

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Immunofluorescence (Frozen): 1:50 - 1:100 Immunofluorescence (Immunocytochemistry): 1:100

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Immunofluorescence (Frozen): 1:800

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Immunofluorescence (Frozen): 1:800 Flow Cytometry (Fixed/Permeabilized): 1:80 Flow Cytometry (Live): 1:80

(1)

Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:20 Flow Cytometry (Live): 1:20

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Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:6400

(1)

Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:400

(1)

Immunohistochemistry (Paraffin): 1:400

(1)

Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:200 Immunofluorescence (Immunocytochemistry): 1:800

(1)

Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:50 Immunofluorescence (Immunocytochemistry): 1:400

(1)

Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:50 Immunofluorescence (Immunocytochemistry): 1:50

(1)

Immunoprecipitation: 1:50 IHC Leica Bond: 1:100 - 1:400 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200

(1)

WB FCM IP Neutralizing /Inhibition

(1)

WB ICC/IF FCM IP

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WB ICC/IF IHC-P

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WB ICC/IF IHC-P IHC-Fr FCM IP Depletion

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WB IHC-P

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WB IHC-P ELISA

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WB IHC-P FCM IP MS

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WB IHC-P IHC-Fr FCM Neutralizing /Inhibition

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WB IP

(1)

Western Blotting: 1:1000 Immunoprecipitation: 1:200

(1)

Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400

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mIHC

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Host

Rat

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Reactivity

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Conjugation

Unconjugated

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Phycoerythrin (PE) Wavelength

(13)

Fluorescein isothiocyanate (FITC) Wavelength

(11)

Allophycocyanin (APC) Wavelength

(8)

Non-conjugated

(7)

Phycoerythrin-Cyanine7 (PE-Cy7) Wavelength

(5)

Biotin

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Peridinin-chlorophyll proteins (PerCP) Wavelength

(4)

Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5) Wavelength

(4)

FITC

(3)

unconjuagate

(3)

Allophycocyanin-Cyanine7 (APC-Cy7) Wavelength

(2)

Biotin The reagent is free of unconjugated biotin.

(2)

Phycoerythrin-Cyanine5 (PE-Cy5) Wavelength

(2)

ABflo® 450. Ex:406nm. Em:445nm.

(1)

ABflo® 488. Ex:491nm. Em:516nm.

(1)

ABflo® 500. Ex:410nm. Em:501nm.

(1)

ABflo® 610. Ex:421nm. Em:612nm.

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AF450

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AF700

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AF780

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APC

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APC-Cy7. Ex:651nm. Em:779nm.

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APC. Ex:650nm. Em:660nm.

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APC/Cy7®

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PE / AAF594

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PE. Ex:565nm. Em:574nm.

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PerCP-Cy5.5. Ex:482nm. Em:695nm.

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PerCP/Cy5.5®

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redFluor™ 710

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violetFluor™ 450

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Clonality

Monoclonal

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Polyclonal

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Monoclonal Antibody

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Multiclonal

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Recombinant

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monoclonal

(1)

Clone Number

RA3-6B2

(94)

ATC10005

(9)

H857

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YA3710

(2)

YTH 80.103

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(#9F14)

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351C5

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ARC5024

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ARC52280

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ARC52283

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ARC54815

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ARC60962

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ARC65145

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HIS24

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IVA103

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QA19A52

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YA4687

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YA5587

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YA6135

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YA6369

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YA6408

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Isotype

Rat IgG2a, κ

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IgG1

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IgG2a

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IgG2b

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IgG

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Rat / IgG2a, kappa

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IgM Kappa

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IgG2

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IgM

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Rat IgG2a

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