Search results for Protein kinase B alpha
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PathScan®Phospho-Akt (Thr308) Chemiluminescent Sandwich ELISA Kit#7135, Cell Signaling Technology (CST), 7135
PathScan®Phospho-Akt (Thr308) Chemiluminescent Sandwich ELISA Kit #7135 detects endogenous levels of phospho-Akt (Thr308) in human and mouse cells. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Mouse
Applications
Conjugation
PathScan®Phospho-Akt (Thr308) Sandwich ELISA Kit#7252, Cell Signaling Technology (CST), 7252
PathScan®Phospho-Akt (Thr308) Sandwich ELISA Kit #7252 detects endogenous levels of phospho-Akt (Thr308) protein. As shown in Figure 1, using this ELISA Kit #7252, a significant induction of phospho-Akt (Thr308) is detected in NIH/3T3 cells treated with PDGF. However, levels of total Akt (phospho and nonphospho) detected by PathScan®Total Akt1 Sandwich ELISA Kit #7170, remain unchanged (Figure 1). Phospho-Akt (Thr308) in Jurkat cells is also detected by this ELISA kit #7252. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Mouse
Applications
Conjugation
PathScan®Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit#7134, Cell Signaling Technology (CST), 7134
PathScan®Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit detects endogenous levels of phospho-Akt1 (Ser473) in human, rat and mouse cells. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Mouse, Rat
Applications
Conjugation
PathScan®Phospho-Akt1 (Ser473) Sandwich ELISA Kit#7160, Cell Signaling Technology (CST), 7160
PathScan®Phospho-Akt1 (Ser473) Sandwich ELISA Kit #7160 detects endogenous levels of phosphorylated Akt1 at Ser473. Inhibition of phosphorylation of Akt1 (Ser473) by LY294002 in Jurkat cells is shown in Figure 1. Treatment of NIH/3T3 cells with PDGF to induce phosphorylation of Akt1 (Ser473) is also shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Mouse, Rat
Applications
Conjugation
PathScan®Total Akt1 Chemiluminescent Sandwich ELISA Kit#7132, Cell Signaling Technology (CST), 7132
PathScan®Total Akt1 Chemiluminescent Sandwich ELISA Kit #7132 detects endogenous levels of total Akt1 protein in human and mouse cells. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Mouse
Applications
Conjugation
PathScan®Total Akt1 Sandwich ELISA Kit#7170, Cell Signaling Technology (CST), 7170
PathScan®Total Akt1 Sandwich ELISA Kit detects endogenous levels of Akt1 including phosphorylated and nonphosphorylated. Inhibition of phosphorylation of Akt at serine 473 by LY294002 in NIH/3T3 cells is detected by PathScan®Phospho-Akt1 (Ser473) Sandwich ELISA Kit #7160, while the level of total Akt1 protein detected by this ELISA kit #7170 is unchanged, as seen in Figure 1. Similar results have also observed in Jurkat cells (data not shown). This ELISA kit does not detect related kinases, such as PKC and p70 S6 kinase. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Mouse
Applications
Conjugation
Akt (5G3) Mouse Monoclonal Antibody#2966, Cell Signaling Technology (CST), 2966
Akt (5G3) Mouse Monoclonal Antibody detects endogenous levels of Akt1 and Akt3. This antibody does not cross-react with other related proteins.
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster
Applications
Immunoprecipitation: 1:100
Conjugation
Akt Antibody#9272, Cell Signaling Technology (CST), 9272
Akt Antibody detects endogenous levels of total Akt1, Akt2 and Akt3 proteins. This antibody does not cross-react with related kinases.
Host
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Chicken, D. melanogaster, Bovine, Dog, Pig, Guinea Pig
Applications
Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200
Conjugation
Akt (E17K Mutant Specific) (D1T7P) Rabbit Monoclonal Antibody#14702, Cell Signaling Technology (CST), 14702
Akt (E17K Mutant Specific) (D1T7P) Rabbit Monoclonal Antibody recognizes levels of transfected E17K mutant Akt1 and Akt2 protein. This antibody weakly recognizes transfected E17K mutant Akt3.
Host
Rabbit
Reactivity
Human
Applications
Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:800
Conjugation
Akt (pan) (11E7) Rabbit Monoclonal Antibody (BSA and Azide Free)#86492, Cell Signaling Technology (CST), 86492
Akt (pan) (11E7) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total Akt protein. This antibody does not cross-react with other related proteins.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Applications
Conjugation
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