Search results for 550

Spark Blue™ 550 anti-human CD4 Antibody, IFN-γR b chain, SK3, BioLegend, 344655

Spark Blue™ 550 anti-human CD4 Antibody, IFN-γR b chain, SK3, BioLegend, 344656

Spark Blue™ 550 anti-human CD31 (Flexi-Fluor™) Antibody, CD31, WM59, BioLegend, 285274

Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.

Spark Blue™ 550 anti-mouse NK1.1 (Flexi-Fluor™) Antibody, NK-1.1, S17016D, BioLegend, 285279

Clone S17016D cross-blocks anti-mouse NK1.1 clone PK136, and can stain for NK1.1 post-formaldehyde and methanol-based fixation based on in-house testing.

Spark Blue™ 550 anti-human CD64 Antibody (Flexi-Fluor™) Antibody, CD64, S18012C, BioLegend, 285270

In-house testing suggests that clones S18012C and 10.1 do not cross-block each other and recognize different epitopes. Clone 10.1 recognizes the EC3 epitope of CD64. While both CD64 subunits contain EC3 domains, clone 10.1 preferentially binds to CD64A (FcγRIA) but not CD64B (FcγRIB).

Spark Blue™ 550 anti-mouse CD154 (CD40L) (Flexi-Fluor™) Antibody, CD154, SA047C3, BioLegend, 285284

Spark Blue™ 550 anti-mouse CD185 (CXCR5) (Flexi-Fluor™) Antibody, CD185, L138D7, BioLegend, 285240

Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.

Spark Blue™ 550 anti-human CD45 Recombinant Antibody (Flexi-Fluor™), CD45, QA17A19, BioLegend, 285293

Clone QA17A19 does not block clones 2D1 and HI30 indicating a unique epitope.

Spark Blue™ 550 anti-human CD154 (Flexi-Fluor™) Antibody, CD154, 24-31, BioLegend, 285246

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1,3and blocking of T cell-dependent B cell differentiation1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Spark Blue™ 550 anti-mouse CD335 (NKp46) (Flexi-Fluor™) Antibody, CD335, 29A1.4, BioLegend, 285247

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2andin vitroactivation of NK cells1.