Search results for 593

Spark YG™ 593 anti-mouse CD69 (Flexi-Fluor™) Antibody, CD69, H1.2F3, BioLegend, 285439

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include:in vitroT cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.This antibody has been characterized in the literature as containing a lambda light chain.

Spark YG™ 593 anti-human CD45RA (Flexi-Fluor™) Antibody, CD45RA, HI100, BioLegend, 285442

Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.

Spark YG™ 593 anti-human CD8a (Flexi-Fluor™) Antibody, CD8a, HIT8a, BioLegend, 285443

Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Spark YG™ 593 anti-mouse CD31 (Flexi-Fluor™) Antibody, CD31, MEC13.3, BioLegend, 285553

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8Additional reported applications (in the relevant formats) include: immunoprecipitation1,in vitroandin vivoblocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.Special Note:The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.102529 and 102530).

Spark YG™ 593 anti-mouse CD11c (Flexi-Fluor™) Antibody, CD11c, N418, BioLegend, 285449

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9(Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Spark YG™ 593 anti-human CD27 (Flexi-Fluor™) Antibody, CD27, O323, BioLegend, 285450

Spark YG™ 593 anti-human CD33 (Flexi-Fluor™) Antibody, CD33, P67.6, BioLegend, 285577

Spark YG™ 593 anti-mouse CD25 (Flexi-Fluor™) Antibody, CD25, PC61, BioLegend, 285451

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,in vitroblocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4,in vivodepletion of CD25+CD4+Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.

Spark YG™ 593 anti-mouse F4/80 Recombinant Antibody, F4/80, QA17A29, BioLegend, 157311

Spark YG™ 593 anti-mouse F4/80 Recombinant Antibody, F4/80, QA17A29, BioLegend, 157312