Search results for 387

Spark UV™ 387 anti-mouse IFN-γ Antibody, IFN-γ, XMG1.2, BioLegend, 505864

ELISA1-4,11,14or ELISPOT5Detection:The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.ELISA or ELISPOT Capture:The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).Flow Cytometry7,8,12,13,16:The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.Neutralization1-3,9,10:The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivityin vivoandin vitro(Cat. No. 505812). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).Additional reported applications (for the relevant formats) include:Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.Note:For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.

Spark UV™ 387 anti-human CD38 (Flexi-Fluor™) Antibody, CD38, S17015F, BioLegend, 285501

S17015F is able to cross-block binding of clones HIT2 and HB-7 also raised against human CD38, but not S17015A based on in-house testing.

Spark UV™ 387 anti-mouse CD86 (Flexi-Fluor™) Antibody, CD86, A17199A, BioLegend, 285509

Clone A17199A is able to completely block the binding of clone PO3 to CD86.

Spark UV™ 387 anti-human CD62L (Flexi-Fluor™) Antibody, CD62L, S21001C, BioLegend, 285502

Clone S21001C does not block the binding of clone DREG-56 on target cells.Clone S21001C may be more sensitive in detecting CD62L surface expression level changes.

Spark UV™ 387 anti-mouse CD31 (Flexi-Fluor™) Antibody, CD31, 390, BioLegend, 285505

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroandin vivoblocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections.Special Note:This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).

Spark UV™ 387 anti-mouse CD317 (BST2, PDCA-1) Antibody, CD317, 927, BioLegend, 127043

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, functional assay2, and depletion3,4. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

Spark UV™ 387 anti-mouse CD317 (BST2, PDCA-1) Antibody, CD317, 927, BioLegend, 127044

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, functional assay2, and depletion3,4. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

Spark UV™ 387 anti-human CD86 (Flexi-Fluor™) Antibody, CD86, BU63, BioLegend, 285488

Spark UV™ 387 anti-human CD28 (Flexi-Fluor™) Antibody, CD28, CD28.2, BioLegend, 285489

The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays.

Spark UV™ 387 anti-human CD69 (Flexi-Fluor™) Antibody, CD69, FN50, BioLegend, 285492

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.