Search results for 550

Spark Blue™ 550 anti-human CD90 (Thy1) (Flexi-Fluor™) Antibody, CD90, 5E10, BioLegend, 285252

Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.

Spark Blue™ 550 anti-human CD193 (CCR3) (Flexi-Fluor™) Antibody, CD193, 5E8, BioLegend, 285003

Additional reported applications (for the relevant formats) include: The 5E8 antibody is useful for immunofluorescent staining and flow cytometric analysis of CCR3 expression.It has been observed that the 5E8 antibody clone can interact with PE/Cyanine7 antibody conjugates during multi-color staining, potentially leading to unwanted staining. This interaction can be resolved by sequentially staining with the 5E8 antibody first and then followed by the PE/Cyanine7 conjugate of interest.

Spark Blue™ 550 anti-human CD335 (NKp46) (Flexi-Fluor™) Antibody, CD335, 9E2, BioLegend, 285253

Clone 9E2 has been shown to block NK activation through NKp46.6

Spark Blue™ 550 anti-mouse CD45RB (Flexi-Fluor™) Antibody, CD45RB, C363-16A, BioLegend, 285287

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Spark Blue™ 550 anti-human CD44 (Flexi-Fluor™) Antibody, CD44, C44Mab-5, BioLegend, 285009

Spark Blue™ 550 anti-human CD163 (Flexi-Fluor™) Antibody, CD163, GHI/61, BioLegend, 285261

Clone GHI/61 binds to domain 7 of CD163. Additional reported applications (for the relevant formats) include: immunocytochemical staining, immunoprecipitation, western blot1, and spatial biology (IBEX)6,7.

Spark Blue™ 550 anti-human/mouse CD49f (Flexi-Fluor™) Antibody, CD49f, GoH3, BioLegend, 285280

Additional reported applications (for the relevant formats) include: immunoprecipitation1,5,in vitroandin vivoblocking of cell binding to laminin and blocking the function of integrin a61,4, and immunohistochemistry of acetone-fixed frozen sections2,3,5. The GoH3 antibody has been reported to block laminin bindingin vitroand to block integrin a6functionin vivo.

Spark Blue™ 550 anti-mouse/human CD44 (Flexi-Fluor™) Antibody, CD44, IM7, BioLegend, 285013

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3,in vivoinhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Spark Blue™ 550 anti-rat CD45 (Flexi-Fluor™) Antibody, CD45, OX-1, BioLegend, 285278

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy (acetone fixed, ammonium-thiocyanate separated epidermal sheets)2, immunohistochemistry of acetone3-, isopentane-4, PLP5-, and n-hexane6fixed frozen sections and paraffin-embedded sections7,8, and partial inhibition of NK cell lysis of syngeneic tumor cell lines1.

Spark Blue™ 550 anti-rat CD45RA (Flexi-Fluor™) Antibody, CD45RA, OX-33, BioLegend, 285285

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1.