Search results for 593

Spark YG™ 593 anti-mouse CX3CR1 (Flexi-Fluor™) Antibody, CX3CR1, SA011F11, BioLegend, 285554

View more applications data for a different format of this clone in ourScientific Poster Library.

Spark YG™ 593 anti-human CD45RO (Flexi-Fluor™) Antibody, CD45RO, UCHL1, BioLegend, 285457

The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.UCHL1 signal intensity may be reduced in the presence of True-Stain Monocyte Blocker™ (Cat. No. 426102). Clone S19021B is not impacted by Monocyte Blocker and is recommended as an alternative to UCHL1.

Spark YG™ 593 anti-human CD235a (Glycophorin A) (Flexi-Fluor™) Antibody, CD235a, HI264, BioLegend, 285563

Spark YG™ 593 anti-mouse CD206 (MMR) (Flexi-Fluor™) Antibody, CD206, C068C2, BioLegend, 285433

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Spark YG™ 593 anti-mouse CD185 (CXCR5) (Flexi-Fluor™) Antibody, CD185, L138D7, BioLegend, 285575

Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.

Spark YG™ 593 anti-human CD314 (NKG2D) (Flexi-Fluor™) Antibody, CD314, 1D11, BioLegend, 285556

The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.

Spark YG™ 593 anti-mouse CD19 (Flexi-Fluor™) Antibody, CD19, 1D3/CD19, BioLegend, 285422

Additional reported applications (for the relevant formats) include: blocking1,2and immunoprecipitation1.

Spark YG™ 593 anti-mouse CD335 (NKp46) (Flexi-Fluor™) Antibody , CD335, 29A1.4, BioLegend, 285559

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2andin vitroactivation of NK cells1.

Spark YG™ 593 anti-mouse CD8a (Flexi-Fluor™) Antibody, CD8a, 53-6.7, BioLegend, 285424

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Spark YG™ 593 anti-human CD90 (Thy1) (Flexi-Fluor™) Antibody, CD90, 5E10, BioLegend, 285569

Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.