Search results for 387
Spark UV™ 387 anti-mouse CD69 (Flexi-Fluor™) Antibody, CD69, H1.2F3, BioLegend, 285494
The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include:in vitroT cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.This antibody has been characterized in the literature as containing a lambda light chain.
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC
Conjugation
Spark UV™ 387 anti-mouse CD31 (Flexi-Fluor™) Antibody, CD31, MEC13.3, BioLegend, 285514
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8Additional reported applications (in the relevant formats) include: immunoprecipitation1,in vitroandin vivoblocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.Special Note:The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.102529 and 102530).
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation
Spark UV™ 387 anti-human IL-2 Antibody, IL-2, MQ1-17H12, BioLegend, 500361
ELISA or ELISPOT Capture2,3:The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 µg/mL is recommended.Additional reported applications (for the relevant formats) include:immunoprecipitation2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,4-6,8, neutralization13, and immunocytochemistry.Note:For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 431807) is specially developed and recommended.Clone MQ1-17H12 cross-reacts to Cat15
Host
Rat
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Spark UV™ 387 anti-human IL-2 Antibody, IL-2, MQ1-17H12, BioLegend, 500362
ELISA or ELISPOT Capture2,3:The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 µg/mL is recommended.Additional reported applications (for the relevant formats) include:immunoprecipitation2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,4-6,8, neutralization13, and immunocytochemistry.Note:For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 431807) is specially developed and recommended.Clone MQ1-17H12 cross-reacts to Cat15
Host
Rat
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Spark UV™ 387 anti-mouse CD25 (Flexi-Fluor™) Antibody, CD25, PC61, BioLegend, 285500
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,in vitroblocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4,in vivodepletion of CD25+CD4+Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation
Spark UV™ 387 anti-mouse CX3CR1 (Flexi-Fluor™) Antibody , CX3CR1, SA011F11, BioLegend, 285516
View more applications data for a different format of this clone in ourScientific Poster Library.
Host
Mouse
Reactivity
Mouse
Applications
FC
Conjugation
Spark UV™ 387 anti-human IgM (Flexi-Fluor™) Antibody, IgM, MHM-88, BioLegend, 285515
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation
Spark UV™ 387 anti-human/mouse Granzyme B Recombinant Antibody, Granzyme B, QA18A28, BioLegend, 396441
Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).
Host
Rat
Reactivity
Human, Mouse
Applications
ICFC - Quality tested
Conjugation
Spark UV™ 387 anti-human/mouse Granzyme B Recombinant Antibody, Granzyme B, QA18A28, BioLegend, 396442
Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).
Host
Rat
Reactivity
Human, Mouse
Applications
ICFC - Quality tested
Conjugation
Spark UV™ 387 anti-human CD95 (Fas) (Flexi-Fluor™) Antibody, CD95, DX2, BioLegend, 285490
The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include:in vitroinduction of apoptosis3(DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656).Note:EOS9.1 antibody can induce apoptosis without cross-linking.
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation
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