Search results for 640
PE/Fire™ 640 anti-mouse CD152 Antibody, CD152, UC10-4B9, BioLegend, 106333
The UC10-4B9 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitrostimulation,in vitroandin vivoblocking1-4of ligand binding, and as ELISA capture antibody5. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106327).
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC - Quality Tested
Conjugation
PE/Fire™ 640 anti-mouse CD152 Antibody, CD152, UC10-4B9, BioLegend, 106334
The UC10-4B9 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitrostimulation,in vitroandin vivoblocking1-4of ligand binding, and as ELISA capture antibody5. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106327).
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC - Quality Tested
Conjugation
PE/Fire™ 640 anti-mouse NK-1.1 Antibody, NK-1.1, S17016D, BioLegend, 156545
Clone S17016D cross-blocks anti-mouse NK1.1 clone PK136, and can stain for NK1.1 post-formaldehyde and methanol-based fixation based on in-house testing.
Host
Mouse
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
PE/Fire™ 640 anti-mouse NK-1.1 Antibody, NK-1.1, S17016D, BioLegend, 156546
Clone S17016D cross-blocks anti-mouse NK1.1 clone PK136, and can stain for NK1.1 post-formaldehyde and methanol-based fixation based on in-house testing.
Host
Mouse
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
PE/Fire™ 640 anti-mouse CD127 (IL-7Rα) Antibody, CD127, S18006K, BioLegend, 158213
Clone S18006K is able to completely block the binding of clone SB/199 and partially block the binding of clone A7R34 to IL-7Rα.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
PE/Fire™ 640 anti-mouse CD127 (IL-7Rα) Antibody, CD127, S18006K, BioLegend, 158214
Clone S18006K is able to completely block the binding of clone SB/199 and partially block the binding of clone A7R34 to IL-7Rα.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
PE/Fire™ 640 anti-human CD279 (PD-1) Antibody, CD279, A17188B, BioLegend, 621623
A17188B antibody can block the binding of NAT105 and EH12.2H7 antibodies to the target.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
PE/Fire™ 640 anti-human CD279 (PD-1) Antibody, CD279, A17188B, BioLegend, 621624
A17188B antibody can block the binding of NAT105 and EH12.2H7 antibodies to the target.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
PE/Fire™ 640 anti-human CD127 (IL-7Rα) Antibody, CD127, A019D5, BioLegend, 351363
Additional reported (for the relevant formats) application: proteogenomics1.
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
PE/Fire™ 640 anti-human CD127 (IL-7Rα) Antibody, CD127, A019D5, BioLegend, 351364
Additional reported (for the relevant formats) application: proteogenomics1.
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
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