Search results for CD25 antibody
TotalSeq™-C0097 anti-mouse CD25 Antibody, CD25, PC61, BioLegend, 102065
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,in vitroblocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4,in vivodepletion of CD25+CD4+Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
Host
Rat
Reactivity
Mouse
Applications
PG -Quality tested
Conjugation
Spark PLUS UV395™ anti-human CD25 Antibody, CD25, S20019C, BioLegend, 385609
Clone S20019C can completely block binding of clone BC96 and clone TÜ69 on target cells and can be blocked by BC96 and by TÜ69.Clone S20019C does not block the binding of clone S20019D and clone M-A251 on target cells.Clone S20019C can block IL-2 binding to receptor.Clone S20019C shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark PLUS UV395™ anti-human CD25 Antibody, CD25, S20019C, BioLegend, 385610
Clone S20019C can completely block binding of clone BC96 and clone TÜ69 on target cells and can be blocked by BC96 and by TÜ69.Clone S20019C does not block the binding of clone S20019D and clone M-A251 on target cells.Clone S20019C can block IL-2 binding to receptor.Clone S20019C shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
APC/Fire™ 750 anti-human CD25 Antibody, CD25, S20019D, BioLegend, 385217
Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251.Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells.Clone S20019D does not affect binding of IL-2 to the receptor.Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
APC/Fire™ 750 anti-human CD25 Antibody, CD25, S20019D, BioLegend, 385218
Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251.Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells.Clone S20019D does not affect binding of IL-2 to the receptor.Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
PE/Dazzle™ 594 anti-human CD25 Antibody, CD25, S20019D, BioLegend, 385223
Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251.Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells.Clone S20019D does not affect binding of IL-2 to the receptor.Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
PE/Dazzle™ 594 anti-human CD25 Antibody, CD25, S20019D, BioLegend, 385224
Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251.Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells.Clone S20019D does not affect binding of IL-2 to the receptor.Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
PE/Fire™ 700 anti-human CD25 Antibody, CD25, S20019D, BioLegend, 385219
Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251.Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells.Clone S20019D does not affect binding of IL-2 to the receptor.Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
PE/Fire™ 700 anti-human CD25 Antibody, CD25, S20019D, BioLegend, 385220
Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251.Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells.Clone S20019D does not affect binding of IL-2 to the receptor.Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-human CD25 Antibody, CD25, S20019D, BioLegend, 385211
Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251.Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells.Clone S20019D does not affect binding of IL-2 to the receptor.Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
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