Search results for IL 10
![Anti-IL-10 antibody [EPR20433-201] - BSA and Azide free (Capture) Brand Logo](https://file.buyantibody.com/public/brand/20260608/Abcam.jpg "Click to View Anti-IL-10 antibody [EPR20433-201] - BSA and Azide free (Capture) Product Detail")
Anti-IL-10 antibody [EPR20433-201] - BSA and Azide free (Capture), Abcam, AB244835
ab244835 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human IL-10. The recommended pair for sandwich ELISA is: Capture: ab244835, Human IL-10 (Interleukin-10) Capture Antibody (unconjugated) Detector: ab244889 , Human IL-10 (Interleukin-10) Detector Antibody (unconjugated) The reference range value is 6.86 - 5000 pg/ml. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Host
Rabbit
Reactivity
Human
Applications
sELISA
Conjugation
Anti-IL-10 antibody [EPR20433-290] - BSA and Azide free (Detector), Abcam, AB244889
ab244889 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human IL-10. The recommended pair for sandwich ELISA is: Capture: ab244835 , Human IL-10 (Interleukin-10) Capture Antibody (unconjugated) Detector: ab244889, Human IL-10 (Interleukin-10) Detector Antibody (unconjugated) The reference range value is 6.86 - 5000 pg/ml. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Host
Rabbit
Reactivity
Human, Monkey
Applications
sELISA
Conjugation
PE anti-mouse CD210 IL-10 R Antibody anti-CD210 - 1B1.3a, BioLegend, 112705
Additional reported applications (for the relevant formats) include:in vitroandin vivoblocking of ligand binding1-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 112706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112704) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 112710) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
PE anti-mouse CD210 IL-10 R Antibody anti-CD210 - 1B1.3a, BioLegend, 112706
Additional reported applications (for the relevant formats) include:in vitroandin vivoblocking of ligand binding1-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 112706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112704) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 112710) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Purified anti-mouse CD210 IL-10 R Antibody anti-CD210 - 1B1.3a, BioLegend, 112702
Additional reported applications (for the relevant formats) include:in vitroandin vivoblocking of ligand binding1-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 112706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112704) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 112710) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
FC -Quality testedBlock -Reported in the literature, not verified in house
Conjugation
Biotin anti-mouse CD210 IL-10 R Antibody anti-CD210 - 1B1.3a, BioLegend, 112704
Additional reported applications (for the relevant formats) include:in vitroandin vivoblocking of ligand binding1-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 112706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112704) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 112710) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Purified anti-human CD210 IL-10 R Antibody anti-CD210 - 3F9, BioLegend, 308802
Clone 3F9 recognizes the IL-10-binding epitope of IL-10R1.8Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroblocking1-3of human IL-10 binding to IL-10R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-rat IgG second step), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays (Cat. No. 308817 and 308818).
Host
Rat
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC -Quality testedIP, Block -Reported in the literature, not verified in house
Conjugation
APC anti-human CD210 IL-10 R Antibody anti-CD210 - 3F9, BioLegend, 308811
Clone 3F9 recognizes the IL-10-binding epitope of IL-10R1.8Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroblocking1-3of human IL-10 binding to IL-10R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-rat IgG second step), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays (Cat. No. 308817 and 308818).
Host
Rat
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
APC anti-human CD210 IL-10 R Antibody anti-CD210 - 3F9, BioLegend, 308812
Clone 3F9 recognizes the IL-10-binding epitope of IL-10R1.8Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroblocking1-3of human IL-10 binding to IL-10R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-rat IgG second step), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays (Cat. No. 308817 and 308818).
Host
Rat
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
PE anti-human CD210 IL-10 R Antibody anti-CD210 - 3F9, BioLegend, 308803
Clone 3F9 recognizes the IL-10-binding epitope of IL-10R1.8Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroblocking1-3of human IL-10 binding to IL-10R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-rat IgG second step), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays (Cat. No. 308817 and 308818).
Host
Rat
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
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