Search results for c-Kit antibody
![Anti-c-Kit antibody [EPR25707-15] - BSA and Azide free (Detector) Brand Logo](https://file.buyantibody.com/public/brand/20260608/Abcam.jpg "Click to View Anti-c-Kit antibody [EPR25707-15] - BSA and Azide free (Detector) Product Detail")
Anti-c-Kit antibody [EPR25707-15] - BSA and Azide free (Detector), Abcam, AB318161
ab318161 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human c-Kit. The recommended pair for sandwich ELISA is: Capture: ab318160 , Human c-Kit Capture Antibody (unconjugated) Detector: ab318161, Human c-Kit Detector Antibody (unconjugated) The reference range value is 15.63-2000 pg/mL. The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay. Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Host
Rabbit
Reactivity
Human
Applications
sELISA
Conjugation
Anti-c-Kit antibody [EPR25707-21] - BSA and Azide free (Capture), Abcam, AB318160
ab318160 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human c-Kit. The recommended pair for sandwich ELISA is: Capture: ab318160, Human c-Kit Capture Antibody (unconjugated) Detector: ab318161 , Human c-Kit Detector Antibody (unconjugated) The reference range value is 15.63-2000 pg/mL. The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay. Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Host
Rabbit
Reactivity
Human
Applications
sELISA
Conjugation
Alexa Fluor® 647 Anti-c-Kit antibody [EPR24708-25], Abcam, AB306534
Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
Host
Rabbit
Reactivity
Mouse, Mouse
Applications
ICC/IF, Flow Cyt
Conjugation
Alexa Fluor® 647
PE/Cyanine7 anti-mouse CD117 (c-kit) Antibody, CD117, S18020H, BioLegend, 161611
S18020H has been tested to block the ligand binding of SCF on c-kit which is determined by the phosphorylation of ERK1/2 in c-kit signaling.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
PE/Cyanine7 anti-mouse CD117 (c-kit) Antibody, CD117, S18020H, BioLegend, 161612
S18020H has been tested to block the ligand binding of SCF on c-kit which is determined by the phosphorylation of ERK1/2 in c-kit signaling.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Pacific Blue™ anti-human CD117 (c-kit) Antibody, S18022G, BioLegend, 375209
Clone S18022G antibody can completely block the binding of clone 104D2 to the target, but it does not block the binding of clone w18195C to the target. Clone S18022G antibody binding to the target is not affected after cells are fixed by 4% PFA.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Pacific Blue™ anti-human CD117 (c-kit) Antibody, S18022G, BioLegend, 375210
Clone S18022G antibody can completely block the binding of clone 104D2 to the target, but it does not block the binding of clone w18195C to the target. Clone S18022G antibody binding to the target is not affected after cells are fixed by 4% PFA.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
GMP APC anti-human CD117 (c-kit) Antibody, CD117, 104D2, BioLegend, 260120
The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.
Host
Mouse
Reactivity
Applications
FC - Quality tested
Conjugation
GMP PE anti-human CD117 (c-kit) Antibody, CD117, 104D2, BioLegend, 260124
The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.
Host
Mouse
Reactivity
Applications
FC - Quality tested
Conjugation
PE/Cyanine7 anti-human CD117 (c-kit) Antibody, CD117, W18195C, BioLegend, 375309
Clone W18195C antibody does not block the binding of clones 104D2 or S18022G to the target. Clone W18195C antibody does not bind to the target after cells are fixed by 4% PFA.
Host
Rat
Reactivity
Human
Applications
FC - Quality tested
Conjugation
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