Search results for 550

Spark Blue™ 550 anti-human TCR Vδ2 (Flexi-Fluor™) Antibody, TCR Vδ2, B6, BioLegend, 285257

Spark Blue™ 550 anti-human HLA-A2 (Flexi-Fluor™) Antibody, HLA-A2, BB7.2, BioLegend, 285258

The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles.Additional reported applications (for the relevant formats) include: immunoprecipitation3.

Spark Blue™ 550 anti-human CD152 (CTLA-4) (Flexi-Fluor™) Antibody, CD152, BNI3, BioLegend, 285259

Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.

Spark Blue™ 550 anti-human CD11b (activated) (Flexi-Fluor™) Antibody , CD11b, CBRM1/5, BioLegend, 285295

The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize "non-activated" CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the a chain. Clone CBRM1/5 binds the I domain, close to the ligand binding site. It recognizes a conformational change of the integrin9.Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking cell adhesion to fibrinogen and ICAM-11. The CBRM1/5 antibody binds specifically to the activated form of CD11b. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301408).

Spark Blue™ 550 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) Antibody, CD326, G8.8, BioLegend, 285260

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Spark Blue™ 550 anti-human CD107b (LAMP-2) (Flexi-Fluor™) Antibody, CD107b, H4B4, BioLegend, 285297

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen glomeruli2and immunofluorescent staining of neutrophils2,3.This antibody is specific to human LAMP-2. Positive control: Hela cells; LAMP-2 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Spark Blue™ 550 anti-human Siglec-9 (Flexi-Fluor™) Antibody, Siglec-9, K8, BioLegend, 285296

Additional reported applications (for the relevant formats) include: immunofluorescence staining2, Western blotting2, immunoprecipitation2, and ELISA3.

Spark Blue™ 550 anti-mouse TCR Vβ11 (Flexi-Fluor™) Antibody, TCR Vβ11, KT11, BioLegend, 285301

Spark Blue™ 550 anti-human HLA-DR (Flexi-Fluor™) Antibody, HLA-DR, L243, BioLegend, 285014

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8,in vitroblocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).

Spark Blue™ 550 anti-mouse I-A/I-E Antibody, I-A/I-E, M5/114.15.2, BioLegend, 107661

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-EkMHC class II alloantigens from mice carrying H-2p,r,q,b,d,uhaplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-AsMHC class II alloantigens.1Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6,in vitroandin vivoblocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).