Search results for 593

Spark YG™ 593 anti-human CD107a (LAMP-1) (Flexi-Fluor™) Antibody, CD107a, H4A3, BioLegend, 285440

Additional reported applications (for the relevant formats) include: Western blotting8, immunohistochemical staining2, immunofluorescence5,7, and immunoprecipitation5.This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Spark YG™ 593 anti-mouse Ly-6C (Flexi-Fluor™) Antibody, Ly-6C, HK1.4, BioLegend, 285444

Clone HK1.4 does not block the binding of clone RB6-8C58.Additional reported applications (for relevant formats of this clone) include:in vitroactivation of T cells1-3and immunohistochemistry of frozen sections4.

Spark YG™ 593 anti-mouse NK-1.1 (Flexi-Fluor™) Antibody, NK-1.1, PK136, BioLegend, 285452

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3,in vivodepletion4,5,9,10, mediation ofin vitroredirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Spark YG™ 593 anti-mouse/human KLRG1 (MAFA) (Flexi-Fluor™) Antibody, KLRG1, 2F1/KLRG1, BioLegend, 285560

For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421™, or APC, are recommended.Additional reported applications (for relevant formats) include: Western Blotting1.

Ultra-LEAF™ Purified anti-mouse CD120a (TNF R Type I/p55) Antibody, CD120a, 55R-593, BioLegend, 113103

The 55R-593 antibody is useful for immunoprecipitation1,in vitroandin vivoreceptor blocking1, and for use as an agonist of TNF-a. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 113103 and 113104).

Ultra-LEAF™ Purified anti-mouse CD120a (TNF R Type I/p55) Antibody, CD120a, 55R-593, BioLegend, 113104

The 55R-593 antibody is useful for immunoprecipitation1,in vitroandin vivoreceptor blocking1, and for use as an agonist of TNF-a. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 113103 and 113104).

Spark YG™ 593 anti-mouse CD279 (PD-1) (Flexi-Fluor™) Antibody, CD279, RMP1-30, BioLegend, 285566

The RMP1-30 antibody does not block the binding of PD-1 to B7-H1 and B7-DC1.

Spark YG™ 593 anti-mouse CD64 (FcγRI) (Flexi-Fluor™) Antibody, CD64, X54-5/7.1, BioLegend, 285460

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Spark YG™ 593 anti-human IgG Fc Recombinant Antibody (Flexi-Fluor™) Antibody, IgG Fc, QA19A42, BioLegend, 285565

Anti-human IgG Fc antibody (clone QA19A42) may cross-react with bovine IgG. Anti-human IgG Fc antibody (clone M1310G05) does not exhibit this cross-reactivity.

Spark YG™ 593 anti-human CD366 (Tim-3) (Flexi-Fluor™) Antibody, CD366 (Tim-3), A18087E, BioLegend, 285428