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ICC

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-Reported in the literature

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FC (QC tested)

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FC - Quality testedCyTOF® - Verified

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FC -Quality testedIHC-P

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IF(ICC)

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IF-P

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IFC

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IHC-Fr ELISA Immunoblotting

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IHC-P - Quality tested

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IHC-P FCM IP

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LCI

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Suggested for Flow Cytometry

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WB FCM ELISA

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WB ICC/IF

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not verified in house

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Bioactivity: FACS

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FC. Capture Ab

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Functional assay

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IHC-P - Quality testedICC

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Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Frozen): 1:200 Immunofluorescence (Immunocytochemistry): 1:800

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Immunohistochemistry (Paraffin): 1:50 - 1:200

(1)

Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200

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PG - Quality tested

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Research in vivo

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WB - Verified

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WB ICC/IF IHC-P FCM

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WB ICC/IF IHC-P IHC-Fr

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WB ICC/IF IHC-P IP ELISA PLA

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Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Live): 1:400

(1)

Western Blotting: 1:1000 Immunoprecipitation: 1:50 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:50 - 1:100 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800

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Conjugation

Unconjugated

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(6)

unconjuagate

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Non-conjugated

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APC

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PerCP

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Allophycocyanin (APC) Wavelength

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Biotin

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Fluorescein isothiocyanate (FITC) Wavelength

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Phycoerythrin (PE) Wavelength

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Phycoerythrin-Cyanine7 (PE-Cy7) Wavelength

(3)

APC. Ex:650nm. Em:660nm.

(2)

FITC

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PE-DyLight594

(2)

PE. Ex:565nm. Em:574nm.

(2)

Peridinin-chlorophyll proteins (PerCP) Wavelength

(2)

ABflo® 450. Ex:406nm. Em:445nm.

(1)

ABflo® 488. Ex:491nm. Em:516nm.

(1)

ABflo® 500. Ex:410nm. Em:501nm.

(1)

ABflo® 610. Ex:421nm. Em:612nm.

(1)

APC-Cy7. Ex:651nm. Em:779nm.

(1)

Biotin The reagent is free of unconjugated biotin.

(1)

PE-Cy7. Ex:565nm. Em:778nm.

(1)

PerCP-Cy5.5. Ex:482nm. Em:695nm.

(1)

Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5) Wavelength

(1)

Phycoerythrin-Cyanine5 (PE-Cy5) Wavelength

(1)

Clonality

Monoclonal

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Recombinant

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Polyclonal

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Monoclonal Antibody

(1)

Recombinant,Monoclonal

(1)

Clone Number

5.1H11

(73)

QA17A16

(53)

HCD56

(49)

MEM-188

(24)

QA18A21

(13)

W22097A

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ARC71418

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LT56

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N901-DM1

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AMC0687

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H796

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SAA2213

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U262

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A19063A

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N901

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39D5

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B-A19

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YA3780

(2)

(#5F27)

(1)

ARC60182

(1)

B159.52

(1)

ERIC-1

(1)

SAA2007

(1)

W22098A

(1)

W22098E

(1)

YA3222

(1)

YA4690

(1)

YA5643

(1)

Isotype

Mouse IgG1, κ

(190)

IgG2a

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IgG

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IgG1

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Mouse IgG2a, κ

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IgG1, kappa

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Rat IgG2a, κ

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Mouse IgG2b, κ

(5)

IgG2a Kappa

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Rabbit IgG.

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IgG2

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IgG2b

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Mouse IgG1

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Mouse IgG2a

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lgG1

(2)

IgG1 Kappa

(1)

IgG1 kappa

(1)

IgG1-kappa

(1)

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