Search results for IL-2
Brilliant Violet 605 anti-human IL-2 Antibody anti-IL-2 - MQ1-17H12, BioLegend, 500332
ELISA or ELISPOT Capture2,3:The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 µg/mL is recommended.Additional reported applications (for the relevant formats) include:immunoprecipitation2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,4-6,8, neutralization13, and immunocytochemistry.Note:For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 431807) is specially developed and recommended.Clone MQ1-17H12 cross-reacts to Cat15
Host
Rat
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Ultra-LEAF™ Purified anti-mouse IL-2 Recombinant Antibody, IL-2, QA19A06, BioLegend, 937903
This recombinant antibody clone is derived from clone JES6-1A12 .
Host
Mouse
Reactivity
Mouse
Applications
Neut - Quality tested
Conjugation
Ultra-LEAF™ Purified anti-mouse IL-2 Recombinant Antibody, IL-2, QA19A06, BioLegend, 937904
This recombinant antibody clone is derived from clone JES6-1A12 .
Host
Mouse
Reactivity
Mouse
Applications
Neut - Quality tested
Conjugation
Ultra-LEAF Purified anti-mouse IL-2 Antibody anti-IL-2 - JES6-1A12, BioLegend, 503705
ELISA Capture1-3or ELISPOT Capture4-6:The purified JES6-1A12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES6-5H4 antibody (Cat. No. 503804) as the detecting antibody and recombinant mouse IL-2 (Cat. No.575409) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 503704).Neutralization:The JES6-1A12 antibody can neutralize the bioactivity of natural or recombinant IL-2. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 503706) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture - Quality testedELISPOT Capture, Neut, IP, WB - Reported in the literature, not verified in house
Conjugation
Ultra-LEAF Purified anti-mouse IL-2 Antibody anti-IL-2 - JES6-1A12, BioLegend, 503706
ELISA Capture1-3or ELISPOT Capture4-6:The purified JES6-1A12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES6-5H4 antibody (Cat. No. 503804) as the detecting antibody and recombinant mouse IL-2 (Cat. No.575409) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 503704).Neutralization:The JES6-1A12 antibody can neutralize the bioactivity of natural or recombinant IL-2. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 503706) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture - Quality testedELISPOT Capture, Neut, IP, WB - Reported in the literature, not verified in house
Conjugation
Ultra-LEAF Purified anti-mouse IL-2 Antibody anti-IL-2 - JES6-1A12, BioLegend, 503707
ELISA Capture1-3or ELISPOT Capture4-6:The purified JES6-1A12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES6-5H4 antibody (Cat. No. 503804) as the detecting antibody and recombinant mouse IL-2 (Cat. No.575409) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 503704).Neutralization:The JES6-1A12 antibody can neutralize the bioactivity of natural or recombinant IL-2. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 503706) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture - Quality testedELISPOT Capture, Neut, IP, WB - Reported in the literature, not verified in house
Conjugation
Ultra-LEAF Purified anti-mouse IL-2 Antibody anti-IL-2 - JES6-1A12, BioLegend, 503708
ELISA Capture1-3or ELISPOT Capture4-6:The purified JES6-1A12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES6-5H4 antibody (Cat. No. 503804) as the detecting antibody and recombinant mouse IL-2 (Cat. No.575409) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 503704).Neutralization:The JES6-1A12 antibody can neutralize the bioactivity of natural or recombinant IL-2. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 503706) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture - Quality testedELISPOT Capture, Neut, IP, WB - Reported in the literature, not verified in house
Conjugation
Ultra-LEAF Purified anti-mouse IL-2 Antibody anti-IL-2 - JES6-1A12, BioLegend, 503709
ELISA Capture1-3or ELISPOT Capture4-6:The purified JES6-1A12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES6-5H4 antibody (Cat. No. 503804) as the detecting antibody and recombinant mouse IL-2 (Cat. No.575409) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 503704).Neutralization:The JES6-1A12 antibody can neutralize the bioactivity of natural or recombinant IL-2. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 503706) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture - Quality testedELISPOT Capture, Neut, IP, WB - Reported in the literature, not verified in house
Conjugation
Ultra-LEAF Purified anti-mouse IL-2 Antibody anti-IL-2 - JES6-1A12, BioLegend, 503710
ELISA Capture1-3or ELISPOT Capture4-6:The purified JES6-1A12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES6-5H4 antibody (Cat. No. 503804) as the detecting antibody and recombinant mouse IL-2 (Cat. No.575409) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 503704).Neutralization:The JES6-1A12 antibody can neutralize the bioactivity of natural or recombinant IL-2. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 503706) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture - Quality testedELISPOT Capture, Neut, IP, WB - Reported in the literature, not verified in house
Conjugation
APC/Fire™ 750 anti-mouse IL-2 Antibody, IL-2, JES6-5H4, BioLegend, 503841
ELISA Detection1-3or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 & 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard.Flow Cytometry8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations.Neutralization1,7: The Ultra-LEAF™ purified antibody (Endotoxinin vivoandin vitro(Cat. No. 503845-503850)) is recommended for neutralization.Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections2,in vivocapture7, and immunocytochemistry.Note:For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. No. 431001 & 431004) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ICFC -Quality tested
Conjugation
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