Search results for 550

Spark Blue™ 550 anti-mouse I-A/I-E Antibody, I-A/I-E, M5/114.15.2, BioLegend, 107662

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-EkMHC class II alloantigens from mice carrying H-2p,r,q,b,d,uhaplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-AsMHC class II alloantigens.1Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6,in vitroandin vivoblocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Spark Blue™ 550 anti-human CD138 (Syndecan-1) (Flexi-Fluor™) Antibody, CD138, MI15, BioLegend, 285273

The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns onin vitrogenerated plasma cells and some CD138+cell lines.4Additional reported applications for the relevant formats include: immunofluorescent staining1, Western blotting2, immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections3, and spatial biology (IBEX)5,6.

Spark Blue™ 550 anti-human CD49e (Flexi-Fluor™) Antibody, CD49e, NKI-SAM-1, BioLegend, 285299

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and inhibition of a5 integrin mediated cell adhesion2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 328004).

Spark Blue™ 550 anti-human CD11c (Flexi-Fluor™) Antibody, CD11c, S-HCL-3, BioLegend, 285001

Additional reported applications (for the relevant formats) include: immunohistochemistry on frozen tissue sections1,2,3,4and immunoprecipitation1.

Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells Antibody, TER-119, TER-119, BioLegend, 116261

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells Antibody, TER-119, TER-119, BioLegend, 116262

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Spark Blue™ 550 anti-human IgG Fc Recombinant Antibody (Flexi-Fluor™), IgG Fc, QA19A42, BioLegend, 285235

Anti-human IgG Fc antibody (clone QA19A42) may cross-react with bovine IgG. Anti-human IgG Fc antibody (clone M1310G05) does not exhibit this cross-reactivity.

Spark Blue™ 550 anti-mouse/human KLRG1 (MAFA) (Flexi-Fluor™) Antibody, KLRG1, 2F1/KLRG1, BioLegend, 285249

For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421™, or APC, are recommended.Additional reported applications (for relevant formats) include: Western Blotting1.

Spark Blue™ 550 anti-human CD328 (Siglec-7) (Flexi-Fluor™) Antibody, CD328, 6-434, BioLegend, 285230

Spark Blue™ 550 anti-human CD169 (Siglec-1) (Flexi-Fluor™) Antibody, CD169, 7-239, BioLegend, 285232

Additional reported applications (for the relevant formats) include: Western blotting and inhibition of erythrocyte-rosetting with cells expressing CD169.