Search results for 593

Spark YG™ 593 anti-mouse CD279 (PD-1) (Flexi-Fluor™) Antibody, PD-1, 29F.1A12, BioLegend, 285423

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3,in vivoblocking of PD-1 binding to its ligands2,3, and spatial biology (IBEX)5,6.

Spark YG™ 593 anti-mouse TCR γ/δ (Flexi-Fluor™) Antibody, TCR γ/δ, GL3, BioLegend, 285562

The GL3 antibody has been shown to be useful in identifying γ/δ T cells by flow cytometry and immunohistochemistry and depleting γ/δ T cellsin vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, andin vivodepletion of γ/δ T cells3-5.

Spark YG™ 593 anti-human TCR α/β (Flexi-Fluor™) Antibody, TCR α/β, IP26, BioLegend, 285445

Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone. Other anti-CD3 clones may compete out the binding of IP26.

Spark YG™ 593 anti-human CD274 (B7-H1, PD-L1) (Flexi-Fluor™) Antibody, CD274, MIH2, BioLegend, 285564

Spark YG™ 593 anti-mouse/human CD45R/B220 (Flexi-Fluor™) Antibody, CD45R/B220, RA3-6B2, BioLegend, 285453

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroandin vivomodulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Spark YG™ 593 anti-human HLA-A,B,C (Flexi-Fluor™) Antibody, HLA-A,B,C, W6/32, BioLegend, 285459

Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Spark YG™ 593 anti-mouse TCR β chain (Flexi-Fluor™) Antibody, TCR β chain, H57-597, BioLegend, 285441

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2,in vitrostimulation2,3,in vivodepletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Spark YG™ 593 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody, I-A/I-E, M5/114.15.2, BioLegend, 285446

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-EkMHC class II alloantigens from mice carrying H-2p,r,q,b,d,uhaplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-AsMHC class II alloantigens.1Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6,in vitroandin vivoblocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Spark YG™ 593 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™) Antibody, TER-119, TER-119, BioLegend, 285456

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Spark YG™ 593 anti-mouse Ly-6A/E (Sca-1) (Flexi-Fluor™) Antibody, Ly-6A/E, D7, BioLegend, 285574

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1,in vitrolymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13and Bouin-fixed, paraffin-embedded samples9.The two Sca-1 recognizing clones D7 andE13-161.7have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14