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PathScan®Total Akt1 Chemiluminescent Sandwich ELISA Kit#7132, Cell Signaling Technology (CST), 7132
PathScan®Total Akt1 Chemiluminescent Sandwich ELISA Kit #7132 detects endogenous levels of total Akt1 protein in human and mouse cells. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Mouse
Applications
Conjugation
PathScan®Total Akt1 Sandwich ELISA Antibody Pair#7142, Cell Signaling Technology (CST), 7142
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan®Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Mouse
Applications
Conjugation
PathScan®Total Akt1 Sandwich ELISA Kit#7170, Cell Signaling Technology (CST), 7170
PathScan®Total Akt1 Sandwich ELISA Kit detects endogenous levels of Akt1 including phosphorylated and nonphosphorylated. Inhibition of phosphorylation of Akt at serine 473 by LY294002 in NIH/3T3 cells is detected by PathScan®Phospho-Akt1 (Ser473) Sandwich ELISA Kit #7160, while the level of total Akt1 protein detected by this ELISA kit #7170 is unchanged, as seen in Figure 1. Similar results have also observed in Jurkat cells (data not shown). This ELISA kit does not detect related kinases, such as PKC and p70 S6 kinase. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Mouse
Applications
Conjugation
Akt (5G3) Mouse Monoclonal Antibody#2966, Cell Signaling Technology (CST), 2966
Akt (5G3) Mouse Monoclonal Antibody detects endogenous levels of Akt1 and Akt3. This antibody does not cross-react with other related proteins.
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster
Applications
Immunoprecipitation: 1:100
Conjugation
Akt Antibody#9272, Cell Signaling Technology (CST), 9272
Akt Antibody detects endogenous levels of total Akt1, Akt2 and Akt3 proteins. This antibody does not cross-react with related kinases.
Host
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Chicken, D. melanogaster, Bovine, Dog, Pig, Guinea Pig
Applications
Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200
Conjugation
Akt (E17K Mutant Specific) (D1T7P) Rabbit Monoclonal Antibody#14702, Cell Signaling Technology (CST), 14702
Akt (E17K Mutant Specific) (D1T7P) Rabbit Monoclonal Antibody recognizes levels of transfected E17K mutant Akt1 and Akt2 protein. This antibody weakly recognizes transfected E17K mutant Akt3.
Host
Rabbit
Reactivity
Human
Applications
Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:800
Conjugation
Akt (pan) (11E7) Rabbit Monoclonal Antibody (BSA and Azide Free)#86492, Cell Signaling Technology (CST), 86492
Akt (pan) (11E7) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total Akt protein. This antibody does not cross-react with other related proteins.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Applications
Conjugation
Akt (pan) (11E7) Rabbit Monoclonal Antibody#4685, Cell Signaling Technology (CST), 4685
Akt (pan) (11E7) Rabbit Monoclonal Antibody detects endogenous levels of total Akt protein. This antibody does not cross-react with other related proteins.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blotting: 1:1000 Simple Western<sup>TM</sup>: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:200 Immunofluorescence (Immunocytochemistry): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:100
Conjugation
Akt (pan) (40D4) Mouse Monoclonal Antibody (BSA and Azide Free)#28544, Cell Signaling Technology (CST), 28544
Akt (pan) (40D4) Mouse Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total Akt protein. This antibody does not cross-react with other related proteins.
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey
Applications
Conjugation
Akt (pan) (40D4) Mouse Monoclonal Antibody#2920, Cell Signaling Technology (CST), 2920
Akt (pan) (40D4) Mouse Monoclonal Antibody detects endogenous levels of total Akt protein. This antibody does not cross-react with other related proteins.
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blotting: 1:2000 Simple Western<sup>TM</sup>: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:250
Conjugation
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