Search results for 550

Spark Blue™ 550 anti-mouse TCR Vγ1.1/Cr4 (Flexi-Fluor™) Antibody, TCR Vγ1.1/Cr4, 2.11, BioLegend, 285224

Additional reported applications (for the relevant formats) include: immunoprecipitation1.

Spark Blue™ 550 anti-human TCR Vα24-Jα18 (Flexi-Fluor™) Antibody, TCR Vα24-Jα18, 6B11, BioLegend, 285264

The 6B11 antibody recognizes the invariant CDR3 region of TCR Va24-JaQ.

Spark Blue™ 550 anti-human HLA-A,B,C (Flexi-Fluor™) Antibody, HLA-A,B,C, W6/32, BioLegend, 285269

Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Spark Blue™ 550 anti-mouse TCR Vβ5.1 5.2 (Flexi-Fluor™) Antibody, TCR Vβ5.1, 5.2, MR9-4, BioLegend, 285282

Additional reported applications (for the relevant formats) include: Induction of proliferation of Vß5.1+and Vß5.2+T cells2, 3andin vivodepletion of Vß5+T cells4.

Spark Blue™ 550 anti-human CD66a/c/e (Flexi-Fluor™) Antibody, CD66a/c/e, ASL-32, BioLegend, 285233

Spark Blue™ 550 anti-mouse TCR β chain (Flexi-Fluor™) Antibody, TCR β chain, H57-597, BioLegend, 285262

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2,in vitrostimulation2,3,in vivodepletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Spark Blue™ 550 anti-mouse TCR Vβ8.1 8.2 (Flexi-Fluor™) Antibody, TCR Vβ8.1, 8.2, KJ16-133.18, BioLegend, 285288

Spark Blue™ 550 anti-rat CD90/mouse CD90.1 (Thy1.1) (Flexi-Fluor™) Antibody, CD90/CD90.1, OX-7, BioLegend, 285271

The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.Additional reported applications (for the relevant formats) include: immunohistochemical7and immunofluorescent8staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation1, Western blotting1,in vitroactivation of leukocytes2, induction of endothelial cell permeability3, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritisin vivo4.

Spark Blue™ 550 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, Ly-6G/Ly-6C, RB6-8C5, BioLegend, 108467

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytesin vivo. Additional reported applications (for relevant formats of this clone) include:in vitrocomplement-mediated cytotoxicity2,in vivodepletion3-5,9, immunoprecipitation1, immunohistochemical staining6(including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.Special Note:Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

Spark Blue™ 550 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, Ly-6G/Ly-6C, RB6-8C5, BioLegend, 108468

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytesin vivo. Additional reported applications (for relevant formats of this clone) include:in vitrocomplement-mediated cytotoxicity2,in vivodepletion3-5,9, immunoprecipitation1, immunohistochemical staining6(including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.Special Note:Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).