Search results for 718
Spark Red™ 718 anti-mouse CD16/32 Antibody, CD16/32, 93, BioLegend, 101350
Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (=1.0 µg per 106cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-human CD152 (CTLA-4) Antibody, CD152, BNI3, BioLegend, 369641
Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality testedFC - Verified
Conjugation
Spark Red™ 718 anti-human CD152 (CTLA-4) Antibody, CD152, BNI3, BioLegend, 369642
Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality testedFC - Verified
Conjugation
Spark Red™ 718 anti-human CD279 (PD-1) Antibody, CD279, EH12.2H7, BioLegend, 329975
Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections13, and spatial biology (IBEX)15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-human CD279 (PD-1) Antibody, CD279, EH12.2H7, BioLegend, 329976
Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections13, and spatial biology (IBEX)15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-human CD107a (LAMP-1) Antibody, CD107a, H4A3, BioLegend, 328659
Additional reported applications (for the relevant formats) include: Western blotting8, immunohistochemical staining2, immunofluorescence5,7, and immunoprecipitation5.This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-human CD107a (LAMP-1) Antibody, CD107a, H4A3, BioLegend, 328660
Additional reported applications (for the relevant formats) include: Western blotting8, immunohistochemical staining2, immunofluorescence5,7, and immunoprecipitation5.This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-mouse Ly-6C Antibody, Ly-6C, HK1.4, BioLegend, 128057
Clone HK1.4 does not block the binding of clone RB6-8C58.Additional reported applications (for relevant formats of this clone) include:in vitroactivation of T cells1-3and immunohistochemistry of frozen sections4.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-mouse Ly-6C Antibody, Ly-6C, HK1.4, BioLegend, 128058
Clone HK1.4 does not block the binding of clone RB6-8C58.Additional reported applications (for relevant formats of this clone) include:in vitroactivation of T cells1-3and immunohistochemistry of frozen sections4.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-mouse CD31 (Flexi-Fluor™), CD31, MEC13.3, BioLegend, 285088
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8Additional reported applications (in the relevant formats) include: immunoprecipitation1,in vitroandin vivoblocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.Special Note:The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.102529 and 102530).
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation
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