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Flow Cytometry (Live): 1:80

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Western Blotting: 1:1000 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:50 - 1:200

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Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:1250 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:100

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Western Blotting: 1:1000 Immunoprecipitation: 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200

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Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:200 Immunofluorescence (Immunocytochemistry): 1:500 Flow Cytometry (Fixed/Permeabilized): 1:50 CUT&RUN: 1:50

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Western Blotting: 1:1000 Immunoprecipitation: 1:50 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:800 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Flow Cytometry (Live): 1:100 - 1:400

(1)

Western Blotting: 1:1000 Simple Western^TM: 1:10 - 1:50 Immunofluorescence (Frozen): 1:1600 - 1:3200 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:12800 Flow Cytometry (Fixed/Permeabilized): 1:1600 - 1:6400 Chromatin IP: 1:50

(1)

Western Blotting: 1:1000 Simple Western^TM: 1:10 - 1:50 Immunoprecipitation: 1:100

(1)

Western Blotting: 1:1000 Simple Western^TM: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:50 - 1:100 Immunofluorescence (Immunocytochemistry): 1:200 - 1:400 Chromatin IP: 1:50

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in vivo study

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Host

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Fluorescein isothiocyanate (FITC) Wavelength

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SignalFlex^TMAlexa Fluor^®532 Conjugate

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SignalFlex^TMAlexa Fluor^®750 Conjugate

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Biotin The reagent is free of unconjugated biotin.

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SignalFlex^TMAlexa Fluor^®350 Conjugate

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SignalFlex^TMmFluor^TMRed 780 Conjugate

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APC

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HRP

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Peridinin-chlorophyll proteins (PerCP) Wavelength

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Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5) Wavelength

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SignalFlex^TMAlexa Fluor^®555 Conjugate

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SignalFlex^TMmFluor^TMUV375 Conjugate

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SignalFlex^TMmFluor^TMViolet 510 Conjugate

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Clonality

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monoclonal

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Clone Number

55R-286

(5)

H398

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PC61/PC61.5.3

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1H4

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55R-170

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55R-593

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PI3KY458-1A11

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SAA0008

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ADCT-301

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EPR27327-41

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EPR27327-79

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PC61

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RM2002

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YA2842

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YA3595

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YA3855

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YA4639

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(#5D31)

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7G7B6

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ARC0320

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ARC1638

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ARC58173

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ARC58302

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ATROSAB

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Clone: 70

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GSK 2862277

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GSK1995057

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SAA1242

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YA1457

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YA2361

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YA4670

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YA5368

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YA5605

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YA6349

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Isotype

IgG

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IgG1

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IgG2a

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IgG1, kappa

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Armenian Hamster IgG

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IgG1, lambda

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IgG2a, kappa

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IgG1-kappa

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IgG2b

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Armenian Hamster IgG1

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IgM

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VHH-8His-Cys-tag

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IgG1 ?

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Mouse IgG2a

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Rat IgG1 Lambda

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VHH-hFc

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dAb (VHH-His)

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