Search results for 581

Spark YG™ 581 anti-human CD71 (Flexi-Fluor™) Antibody, CD71, CY1G4, BioLegend, 285529

Spark YG™ 581 anti-mouse CD4 (Flexi-Fluor™) Antibody, CD4, GK1.5, BioLegend, 285391

Additional reported applications (for the relevant formats) include: blocking of CD4+T cell activation1,4,11, thymocyte costimulation3,in vitroandin vivodepletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Spark YG™ 581 anti-mouse CD69 (Flexi-Fluor™) Antibody, CD69, H1.2F3, BioLegend, 285393

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include:in vitroT cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.This antibody has been characterized in the literature as containing a lambda light chain.

Spark YG™ 581 anti-human CD45 (Flexi-Fluor™) Antibody, CD45, HI30, BioLegend, 285397

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Spark YG™ 581 anti-human CD3 (Flexi-Fluor™) Antibody, CD3, HIT3a, BioLegend, 285398

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Spark YG™ 581 anti-human CD8a (Flexi-Fluor™) Antibody, CD8a, HIT8a, BioLegend, 285399

Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Spark YG™ 581 anti-mouse CD31 (Flexi-Fluor™) Antibody, CD31, MEC13.3, BioLegend, 285520

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8Additional reported applications (in the relevant formats) include: immunoprecipitation1,in vitroandin vivoblocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.Special Note:The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.102529 and 102530).

Spark YG™ 581 anti-mouse CD11c (Flexi-Fluor™) Antibody, CD11c, N418, BioLegend, 285406

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9(Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Spark YG™ 581 anti-human CD27 (Flexi-Fluor™) Antibody, CD27, O323, BioLegend, 285407

Spark YG™ 581 anti-mouse CX3CR1 (Flexi-Fluor™) Antibody, CX3CR1, SA011F11, BioLegend, 285522

View more applications data for a different format of this clone in ourScientific Poster Library.