Search results for Histone H3/f
PathScan®Mono-Methyl-Histone H3 (Lys4) Sandwich ELISA Kit#7123, Cell Signaling Technology (CST), 7123
PathScan®Mono-Methyl-Histone H3 (Lys4) Sandwich ELISA Kit #7123 detects endogenous levels of histone H3 when mono-methylated at Lys4. As shown in Figure 1 using this kit, a high level of mono-methylation at Lys4 on histone H3 is detected in COS cells when treated with TSA. The level of total histone H3 (modified and unmodified) remains unchanged as shown by Western analysis (Figure 1). Similar results are obtained when NIH/3T3 and Jurkat cells are treated with TSA (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human
Applications
Conjugation
PathScan®Phospho-Histone H3 (Ser10) Sandwich ELISA Kit#7155, Cell Signaling Technology (CST), 7155
PathScan®Phospho-Histone H3 (Ser10) Sandwich ELISA Kit detects endogenous levels of Phospho-Histone H3 (Ser10). Using this Sandwich ELISA Kit #7155, Phospho-Histone H3 (Ser10) is detected when treated with Calyculin A in NIH/3T3 cells. However, the levels of Histone H3 remains unchanged, as shown by western analysis using the Histone H3 Antibody #9715 (Figure 1). 293 cells treated with Calyculin A show similar results (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Mouse
Applications
Conjugation
PathScan®RP MG-H1 Glycated Histone H3 (Arg17) Sandwich ELISA Kit#32428, Cell Signaling Technology (CST), 32428
Methylglyoxal-derived hydroimidazolone 1 (MG-H1) is the most predominant of three primary methylglyoxal-derived hydroimidazolone isomers (MG-H1, MG-H2, and MG-H3) that form as advanced glycation end-products when methylglyoxal (MGO) non-enzymatically reacts with arginine residues on proteins.The PathScan®RP MG-H1 Glycated Histone H3 (Arg17) Sandwich ELISA Kit detects endogenous MGO-derived glycation of the MG-H1 subtype at Arg17 of histone H3. This kit may also weakly detect MG-H2 glycated Arg17 of histone H3. In addition, this kit may weakly detect an additional MG-H1 glycated arginine residue on histone H3. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Mouse
Applications
Conjugation
PathScan®RP Mono-Methyl-Histone H3 (Lys9) Sandwich ELISA Kit#38818, Cell Signaling Technology (CST), 38818
The PathScan®RP Mono-Methyl-Histone H3 (Lys9) Sandwich ELISA Kit detects endogenous levels of histone H3 protein when mono-methylated at Lys9. This kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Mouse, Rat, Monkey
Applications
Conjugation
PathScan®Total Histone H3 Sandwich ELISA Kit#7253, Cell Signaling Technology (CST), 7253
PathScan®Total Histone H3 Sandwich ELISA Kit #7253 detects endogenous levels of histone H3. As shown in Figure 1, using this Sandwich ELISA Kit #7253, a high level of histone H3 is detected in NIH/3T3 cells when treated with TSA. The level of total histone H3 (modified and unmodified) remains unchanged as shown by Western analysis (Figure 1). Similar results are obtained when COS and Jurkat cells are treated with TSA (data not shown). Note: For this assay, it is recommended that lysates be thoroughly sonicated to ensure complete extraction of histone H3 and an accurate absorbance reading. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Mouse, Monkey
Applications
Conjugation
PathScan®Tri-Methyl-Histone H3 (Lys4) Sandwich ELISA Kit#7125, Cell Signaling Technology (CST), 7125
PathScan®Tri-Methyl-Histone H3 (Lys4) Sandwich ELISA Kit #7125 detects endogenous levels of histone H3 when tri-methylated at Lys4. As shown in Figure 1 using this kit, a high level of tri-methylation at Lys4 on histone H3 is detected in COS cells when treated with TSA. The level of total histone H3 (modified and unmodified) remains unchanged as shown by Western analysis (Figure 1). Similar results are obtained when NIH/3T3 and Jurkat cells are treated with TSA (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human
Applications
Conjugation
Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody#9711, Cell Signaling Technology (CST), 9711
Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody detects endogenous levels of histone H3 only when modified both by acetylation at lysine 9 and phosphorylation at serine 10.
Host
Reactivity
Human, Mouse, Rat
Applications
Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50
Conjugation
Acetyl-Histone H3 (Lys14) (D4B9) Rabbit Monoclonal Antibody (BSA and Azide Free)#80697, Cell Signaling Technology (CST), 80697
Acetyl-Histone H3 (Lys14) (D4B9) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of Histone H3 protein only when acetylated at Lys14. This antibody does not cross react with histone H3 acetylated at Lys9, 18, 27, or 56.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Applications
Conjugation
Acetyl-Histone H3 (Lys14) (D4B9) Rabbit Monoclonal Antibody#7627, Cell Signaling Technology (CST), 7627
Acetyl-Histone H3 (Lys14) (D4B9) Rabbit Monoclonal Antibody recognizes endogenous levels of Histone H3 protein only when acetylated at Lys14. This antibody does not cross react with histone H3 acetylated at Lys9, 18, 27, or 56.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blotting: 1:1000 Simple Western<sup>TM</sup>: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400 Flow Cytometry (Fixed/Permeabilized): 1:1600 - 1:6400 Chromatin IP: 1:50 Chromatin IP-seq: 1:50
Conjugation
Acetyl-Histone H3 (Lys18) Antibody#9675, Cell Signaling Technology (CST), 9675
Acetyl-Histone H3 (Lys18) Antibody detects endogenous levels of histone H3 only when acetylated at Lys18. This antibody does not cross-react with other acetylated histones.
Host
Reactivity
Human, Mouse, Rat
Applications
Western Blotting: 1:2000 Immunohistochemistry (Paraffin): 1:800 Chromatin IP: 1:25
Conjugation
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