Search results for Anti-Neurofilament H
Alexa Fluor 594 anti-Neurofilament H NF-H Nonphosphorylated Antibody anti-Neurofilament H - SMI 32, BioLegend, 801709
Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+uptake.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedICC, IHC-F -Verified
Conjugation
Alexa Fluor 594 anti-Neurofilament H NF-H Nonphosphorylated Antibody anti-Neurofilament H - SMI 32, BioLegend, 801710
Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+uptake.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedICC, IHC-F -Verified
Conjugation
Purified anti-Neurofilament H NF-H, Nonphosphorylated Antibody anti-Neurofilament H (NF-H) - SMI 32, BioLegend, 801702
Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+uptake.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB -VerifiedArray Tomography, ICC -Reported in the literature, not verified in houseSB -Community verified
Conjugation
Purified anti-Neurofilament H NF-H, Nonphosphorylated Antibody anti-Neurofilament H (NF-H) - SMI 32, BioLegend, 801701
Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+uptake.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB -VerifiedArray Tomography, ICC -Reported in the literature, not verified in houseSB -Community verified
Conjugation
Purified anti-Neurofilament H NF-H, Phosphorylated Antibody anti-Neurofilament H (NF-H), Phospho - SMI 36, BioLegend, 835704
SMI 36 reacts with a phosphorylated epitope in neurofilament H in a variety of mammalian species. SMI 36 reacts with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Like SMI 35, it detects a rich network of thin neuronal cell processes in tissue sections. In the rat brain, nerve cell bodies are unreactive. Other cells and tissues are unreactive except for peripheral axons. Unlike the other antibodies to phosphorylated neurofilament epitopes, SMI 36 does not react with aluminium tangles. Reaction with some cell bodies, such as C3 neurons in the hippocampus, is seen in normal human brain.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB -Verified
Conjugation
Purified anti-Neurofilament H NF-H, Phosphorylated Antibody anti-Neurofilament H (NF-H), Phospho - SMI 36, BioLegend, 835703
SMI 36 reacts with a phosphorylated epitope in neurofilament H in a variety of mammalian species. SMI 36 reacts with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Like SMI 35, it detects a rich network of thin neuronal cell processes in tissue sections. In the rat brain, nerve cell bodies are unreactive. Other cells and tissues are unreactive except for peripheral axons. Unlike the other antibodies to phosphorylated neurofilament epitopes, SMI 36 does not react with aluminium tangles. Reaction with some cell bodies, such as C3 neurons in the hippocampus, is seen in normal human brain.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB -Verified
Conjugation
Purified anti-Neurofilament H (NF-H), Phosphorylated - SMI 34, BioLegend, 835504
SMI 34 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, as well as in chicken. Immunohistochemically, SMI 34 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 34. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 34 may be found also in neuronal cell bodies. In Alzheimer's disease, SMI 34 reacts with intraneuronal tangles but does not react with extraneuronal (ghost) tangles. It is the only anti-phosphoneurofilament that reacts regularly with Alzheimer tangles. It does not cross-react at concentrations of 1:20,000 with Alzheimer MAP-Tau in immunoblots, demonstrating, thereby, the presence of neurofilaments in Alzheimer tangles.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB -Verified
Conjugation
Purified anti-Neurofilament H (NF-H), Phosphorylated - SMI 34, BioLegend, 835503
SMI 34 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, as well as in chicken. Immunohistochemically, SMI 34 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 34. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 34 may be found also in neuronal cell bodies. In Alzheimer's disease, SMI 34 reacts with intraneuronal tangles but does not react with extraneuronal (ghost) tangles. It is the only anti-phosphoneurofilament that reacts regularly with Alzheimer tangles. It does not cross-react at concentrations of 1:20,000 with Alzheimer MAP-Tau in immunoblots, demonstrating, thereby, the presence of neurofilaments in Alzheimer tangles.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB -Verified
Conjugation
Anti-Neurofilament H & M NF-H/NF-M, Hypophosphorylated Antibody anti-Neurofilament H & M (NF-H/NF-M), Phospho - SMI 35, BioLegend, 835601
Additional reported applications (for the relevant formats of this clone): include ELISA Capture1-3, immunohistochemical staining on frozen tissue sections, immunofluorescence staining, and spatial biology (IBEX)6,7.Clone SMI 35 reacts with highly phosphorylated neurofilaments, as well as with low degrees of phosphorylation. It primarily reacts with neurofilament H and with neurofilament M to a lesser extent.Notes: On two dimensional gels, this antibody detects a band extending from the phosphorylated neurofilament position at 200 kD (pI 5.1) toward the non-phosphorylated position at 170 kD (pI 6.2).
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB -VerifiedIHC-F, ELISA -Reported in the literature, not verified in house
Conjugation
Anti-Neurofilament H & M NF-H/NF-M, Hypophosphorylated Antibody anti-Neurofilament H & M (NF-H/NF-M), Phospho - SMI 35, BioLegend, 835602
Additional reported applications (for the relevant formats of this clone): include ELISA Capture1-3, immunohistochemical staining on frozen tissue sections, immunofluorescence staining, and spatial biology (IBEX)6,7.Clone SMI 35 reacts with highly phosphorylated neurofilaments, as well as with low degrees of phosphorylation. It primarily reacts with neurofilament H and with neurofilament M to a lesser extent.Notes: On two dimensional gels, this antibody detects a band extending from the phosphorylated neurofilament position at 200 kD (pI 5.1) toward the non-phosphorylated position at 170 kD (pI 6.2).
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedWB -VerifiedIHC-F, ELISA -Reported in the literature, not verified in house
Conjugation
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