Search results for Ki-67 antibody (Ki-67)

PerCP/Cyanine5.5 anti-mouse/human Ki-67 Antibody, Ki-67, 11F6, BioLegend, 151221

PerCP/Cyanine5.5 anti-mouse/human Ki-67 Antibody, Ki-67, 11F6, BioLegend, 151222

StarBright UltraViolet 740 anti-human Ki-67 antibody, Ki-67, Ki-67, BioLegend, 350542

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.

Pacific Blue™ anti-mouse/human Ki-67 Antibody, Ki-67, 11F6, BioLegend, 151223

Pacific Blue™ anti-mouse/human Ki-67 Antibody, Ki-67, 11F6, BioLegend, 151224

APC/Fire™ 750 anti-mouse Ki-67 antibody, Ki-67, 16A8, BioLegend, 652429

APC/Fire™ 750 anti-mouse Ki-67 antibody, Ki-67, 16A8, BioLegend, 652430

Spark NIR™ 685 anti-mouse Ki-67 Antibody, Ki-67, 16A8, BioLegend, 652431

Spark NIR™ 685 anti-mouse Ki-67 Antibody, Ki-67, 16A8, BioLegend, 652432

Spark PLUS UV395™ anti-human Ki-67 Antibody, Ki-67, Ki-67, BioLegend, 350543

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.