Search results for 581

Spark YG™ 581 anti-human CD3 (Flexi-Fluor™) Antibody, CD3, SK7, BioLegend, 285411

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.

Spark YG™ 581 anti-human CD45RO (Flexi-Fluor™) Antibody, CD45RO, UCHL1, BioLegend, 285413

The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.UCHL1 signal intensity may be reduced in the presence of True-Stain Monocyte Blocker™ (Cat. No. 426102). Clone S19021B is not impacted by Monocyte Blocker and is recommended as an alternative to UCHL1.

Spark YG™ 581 anti-human CD3 (Flexi-Fluor™) Antibody, CD3, UCHT1, BioLegend, 285414

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Spark YG™ 581 anti-human CD235a (Glycophorin A) (Flexi-Fluor™) Antibody, CD235a, HI264, BioLegend, 285532

Spark YG™ 581 anti-mouse CD206 (MMR) (Flexi-Fluor™) Antibody, CD206, C068C2, BioLegend, 285386

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Spark YG™ 581 anti-mouse CD185 (CXCR5) (Flexi-Fluor™) Antibody, CD185, L138D7, BioLegend, 285546

Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.

Spark YG™ 581 anti-human IgM (Flexi-Fluor™) Antibody, IgM, MHM-88, BioLegend, 285521

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Spark YG™ 581 anti-mouse CD3ε (Flexi-Fluor™) Antibody, CD3ε, 145-2C11, BioLegend, 285373

Clone 145-2C11 is useful forin vitroblocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32, andin vivoT cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6,in vitroandin vivostimulation of T cells1,2,7,12,16, immunofluorescent staining5, andin vivoT cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Spark YG™ 581 anti-human CD314 (NKG2D) (Flexi-Fluor™) Antibody, CD314, 1D11, BioLegend, 285524

The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.

Spark YG™ 581 anti-mouse CD335 (NKp46) (Flexi-Fluor™) Antibody, CD335, 29A1.4, BioLegend, 285527

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2andin vitroactivation of NK cells1.