Search results for 581
Spark YG™ 581 anti-mouse CD45 (Flexi-Fluor™) Antibody, CD45, 30-F11, BioLegend, 285378
Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation
Spark YG™ 581 anti-mouse CD8a (Flexi-Fluor™) Antibody, CD8a, 53-6.7, BioLegend, 285380
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation
Spark YG™ 581 anti-human CD90 (Thy1) (Flexi-Fluor™) Antibody, CD90, 5E10, BioLegend, 285539
Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation
Spark YG™ 581 anti-human CD95 (Fas) (Flexi-Fluor™) Antibody, CD95, DX2, BioLegend, 285388
The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include:in vitroinduction of apoptosis3(DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656).Note:EOS9.1 antibody can induce apoptosis without cross-linking.
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation
Spark YG™ 581 anti-human CD163 (Flexi-Fluor™) Antibody, CD163, GHI/61, BioLegend, 285530
Clone GHI/61 binds to domain 7 of CD163. Additional reported applications (for the relevant formats) include: immunocytochemical staining, immunoprecipitation, western blot1, and spatial biology (IBEX)6,7.
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC
Conjugation
Spark YG™ 581 anti-mouse CD86 (Flexi-Fluor™) Antibody, CD86, GL-1, BioLegend, 285392
The GL-1 antibody can block the mixed lymphocyte reactionin vitroand has been shown to inhibit the priming of cytotoxic T lymphocytesin vivo(along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, andin vivoandin vitroblocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation
Spark YG™ 581 anti-mouse/human CD44 (Flexi-Fluor™) Antibody, CD44, IM7, BioLegend, 285401
Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3,in vivoinhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.
Host
Rat
Reactivity
Mouse, Human
Applications
FC
Conjugation
Spark YG™ 581 anti-human CD27 (Flexi-Fluor™) Antibody, CD27, M-T271, BioLegend, 285547
Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections1, immunofluorescent staining2, and ELISA3.
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation
Spark YG™ 581 anti-mouse CD62L (Flexi-Fluor™) Antibody, CD62L, MEL-14, BioLegend, 285405
Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4,in vivoandin vitroblocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation
Spark YG™ 581 anti-mouse/human CD45R/B220 Antibody, CD45R/B220, RA3-6B2, BioLegend, 103281
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroandin vivomodulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.
Host
Rat
Reactivity
Mouse, Human
Applications
FC - Quality tested
Conjugation
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