Search results for IFN-gamma

GoInVivo™ Purified anti-mouse IFN-gamma anti-IFN - XMG1.2, BioLegend, 505851
ELISA1-4,11,14or ELISPOT5Detection:The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.ELISA or ELISPOT Capture:The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).Flow Cytometry7,8,12,13,16:The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.Neutralization1-3,9,10:The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivityin vivoandin vitro(Cat. No. 505812). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).Additional reported applications (for the relevant formats) include:Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.Note:For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture, ELISPOT Capture -Quality testedCyTOF® -VerifiedNeut, ICFC, IHC, WB -Reported in the literature, not verified in house
Conjugation

GoInVivo™ Purified anti-mouse IFN-gamma anti-IFN - XMG1.2, BioLegend, 505852
ELISA1-4,11,14or ELISPOT5Detection:The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.ELISA or ELISPOT Capture:The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).Flow Cytometry7,8,12,13,16:The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.Neutralization1-3,9,10:The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivityin vivoandin vitro(Cat. No. 505812). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).Additional reported applications (for the relevant formats) include:Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.Note:For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture, ELISPOT Capture -Quality testedCyTOF® -VerifiedNeut, ICFC, IHC, WB -Reported in the literature, not verified in house
Conjugation

GoInVivo™ Purified anti-mouse IFN-gamma anti-IFN - XMG1.2, BioLegend, 505853
ELISA1-4,11,14or ELISPOT5Detection:The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.ELISA or ELISPOT Capture:The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).Flow Cytometry7,8,12,13,16:The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.Neutralization1-3,9,10:The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivityin vivoandin vitro(Cat. No. 505812). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).Additional reported applications (for the relevant formats) include:Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.Note:For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture, ELISPOT Capture -Quality testedCyTOF® -VerifiedNeut, ICFC, IHC, WB -Reported in the literature, not verified in house
Conjugation

GoInVivo™ Purified anti-mouse IFN-gamma anti-IFN - XMG1.2, BioLegend, 505854
ELISA1-4,11,14or ELISPOT5Detection:The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.ELISA or ELISPOT Capture:The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).Flow Cytometry7,8,12,13,16:The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.Neutralization1-3,9,10:The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivityin vivoandin vitro(Cat. No. 505812). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).Additional reported applications (for the relevant formats) include:Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.Note:For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture, ELISPOT Capture -Quality testedCyTOF® -VerifiedNeut, ICFC, IHC, WB -Reported in the literature, not verified in house
Conjugation

GoInVivo™ Purified anti-mouse IFN-gamma anti-IFN - XMG1.2, BioLegend, 505855
ELISA1-4,11,14or ELISPOT5Detection:The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.ELISA or ELISPOT Capture:The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).Flow Cytometry7,8,12,13,16:The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.Neutralization1-3,9,10:The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivityin vivoandin vitro(Cat. No. 505812). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).Additional reported applications (for the relevant formats) include:Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.Note:For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture, ELISPOT Capture -Quality testedCyTOF® -VerifiedNeut, ICFC, IHC, WB -Reported in the literature, not verified in house
Conjugation

GoInVivo™ Purified anti-mouse IFN-gamma anti-IFN - XMG1.2, BioLegend, 505856
ELISA1-4,11,14or ELISPOT5Detection:The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.ELISA or ELISPOT Capture:The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).Flow Cytometry7,8,12,13,16:The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.Neutralization1-3,9,10:The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivityin vivoandin vitro(Cat. No. 505812). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).Additional reported applications (for the relevant formats) include:Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.Note:For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture, ELISPOT Capture -Quality testedCyTOF® -VerifiedNeut, ICFC, IHC, WB -Reported in the literature, not verified in house
Conjugation

Ultra-LEAF Purified anti-human IFN-gamma Antibody, IFN-γ, NIB42, BioLegend, 502405
ELISA or ELISPOT Capture2:The purified NIB42 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated 4S.B3 antibody (Cat. No. 502504/502514) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5 - 2.0 µg/ml is recommended.Neutralization1:The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-? bioactivity (Cat. No. 502405 & 502406).Note:For testing human IFN-? in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.
Host
Mouse
Reactivity
Human
Applications
ELISA Capture -Quality testedELISPOT Capture, Neut -Reported in the literature, not verified in house
Conjugation

Ultra-LEAF Purified anti-human IFN-gamma Antibody, IFN-γ, NIB42, BioLegend, 502406
ELISA or ELISPOT Capture2:The purified NIB42 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated 4S.B3 antibody (Cat. No. 502504/502514) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5 - 2.0 µg/ml is recommended.Neutralization1:The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-? bioactivity (Cat. No. 502405 & 502406).Note:For testing human IFN-? in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.
Host
Mouse
Reactivity
Human
Applications
ELISA Capture -Quality testedELISPOT Capture, Neut -Reported in the literature, not verified in house
Conjugation

ABflo® 647 Rabbit anti-Mouse CD119/IFN-gamma receptor 1 mAb, ABclonal, A28265
Host
Reactivity
Mouse
Applications
FC
Conjugation
ABflo® 647. Ex:648nm. Em:664nm.

anti-human IFN gamma Receptor 1 monoclonal antibody Anti-Human 100 µg, Reliatech, 101-M513
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant protein of INFg RI extracellular domain (also called CD119).
Host
Mouse
Reactivity
Human
Applications
WB, FC, N
Conjugation
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