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FC - Quality tested

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(48)

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not verified in house

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Research in vivo

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Block - Reported in the literature

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FC - Quality testedBlock - Reported in the literature

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mIHC

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PG -Quality tested

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FC -Quality testedBlock -Reported in the literature

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sELISA

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ICC

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FC -Quality testedIHC-F -VerifiedBlock -Reported in the literature

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FC -Quality testedIHC-P -VerifiedBlock -Reported in the literature

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I-ELISA

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Immunohistochemistry (Paraffin): 1:100 - 1:400

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Inhibition

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Neutralization

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WB ICC/IF FCM

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in vitro PD-L1 blockade

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in vivo PD-L1 blockade

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FACS

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FC - Quality testedCyTOF® - Verified

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FC - Qualitytested

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FCM Neutralizing /Inhibition

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Flow Cytometry (Fixed/Permeabilized): 1:50 Flow Cytometry (Live): 1:100

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Flow Cytometry (Fixed/Permeabilized): 1:50 Flow Cytometry (Live): 1:50

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Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Flow Cytometry (Live): 1:400 - 1:1600

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Functional assay

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ICC/IF IHC-P IHC-Fr FCM IP

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IHC-P - Quality testedFC - Verified

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IHC-P -Quality tested

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Immunofluorescence (Immunocytochemistry): 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 Flow Cytometry (Live): 1:50

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Immunofluorescence (Immunocytochemistry): 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Flow Cytometry (Live): 1:50 - 1:200

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Immunofluorescence (Immunocytochemistry): 1:200 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 Flow Cytometry (Live): 1:50

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Immunofluorescence (Immunocytochemistry): 1:400 - 1:800

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Immunohistochemistry (Paraffin): 1:50 - 1:200

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Research in vivo

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WB ICC/IF IHC-P FCM

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WB ICC/IF IHC-P IHC-Fr FCM

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WB IHC-P FCM ELISA IHC

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Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200

(1)

Western Blotting: 1:1000 Immunoprecipitation: 1:50 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800

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Western Blotting: 1:1000 Simple Western^TM: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400

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Reactivity

Human

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Mouse

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Plant

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Unconjugated

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Non-conjugated

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Alexa Fluor® 647

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Alexa Fluor® 555

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Alexa Fluor® 568

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HRP

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ABflo® 450. Ex:406nm. Em:445nm.

(2)

ABflo® 488. Ex:491nm. Em:516nm.

(2)

ABflo® 594. Ex:588nm. Em:604nm.

(2)

ABflo® 647. Ex:648nm. Em:664nm.

(2)

APC

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Biotin

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FITC

(2)

PE. Ex:565nm. Em:574nm.

(2)

ABflo® 700. Ex:690nm. Em:713nm.

(1)

Alexa Fluor® 750

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Allophycocyanin (APC) Wavelength

(1)

PE-Cy7. Ex:565nm. Em:778nm.

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PerCP/Cy5.5®

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Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5) Wavelength

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Clonality

Monoclonal

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Polyclonal

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Multiclonal

(2)

Recombinant,Monoclonal

(2)

Monoclonal Antibody

(1)

Clone Number

10F.9G2

(56)

29E.2A3

(56)

MIH3

(20)

28-8

(14)

MIH6

(13)

MIH2

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CAL10

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SP142

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ARC5128-01

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EPR19759

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73-10

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22C3

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ARC61951

(5)

MIH7

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SAA0268

(5)

SAA0359

(4)

EPR23546-160

(3)

EPR20529

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RM1012

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SAA0088

(2)

W20151E

(2)

W20262F

(2)

YA4539

(2)

YA6443

(2)

10F.9G2 / G6-31

(1)

2.7A4

(1)

ABM4E54

(1)

ARC2478

(1)

ARC5146-01

(1)

BLR020E

(1)

EPR23546-147

(1)

EPR23939-25

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IB6

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Iv0226

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Iv0234

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Iv0242

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Iv0244

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Iv0245

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Iv0246

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MIH5

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PRO304397

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Pm8002

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SAA0142

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SAA1141

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SAA1177

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SP263

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Tqb2858

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YA235

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YA4674

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YA6376

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YA6699

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YA8851

(1)

Isotype

IgG

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Mouse IgG2b, κ

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Rat IgG2b, κ

(49)

Mouse IgG1, κ

(32)

Rat IgG2a, λ

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IgG2b

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IgG1

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IgG2a

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IgG1, kappa

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Rat IgG2a, κ

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VHH, mFc

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IgG1-lambda

(2)

IgG2a, lambda

(2)

IgG4, kappa

(2)

IgG4-kappa

(2)

VHH-8His-Cys-tag

(2)

(G1_L-kappa)_VH-VH-h-CH2-CH3

(1)

(VH-CH2-CH3)2

(1)

Human IgG-TGF-β RII

(1)

Human IgG1 Kappa

(1)

Ig VH-VH'-G1(h-CH2-CH3)

(1)

Ig(G1-kappa_G1-lambda2)

(1)

IgG-VHH

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IgG1 (D265A)

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IgG1-kappa

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IgG1-scFv_L-kappa dimer

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IgG1-scFv_L2 dimer

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IgG1;Lambda;Kappa

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IgG2

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IgG2a (D265A), kappa

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IgG2a, kappa

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IgG4

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IgG4, Kappa

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Mouse IgG1

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Mouse IgG2a

(1)

Mouse IgG2b

(1)

Rat IgG2b Kappa

(1)

Single Domains (VH-VH'-CH), IgG1na;na

(1)

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