Search results for 581

Spark YG™ 581 anti-mouse/human CD45R/B220 Antibody, CD45R/B220, RA3-6B2, BioLegend, 103282

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroandin vivomodulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Spark YG™ 581 anti-mouse CD4 (Flexi-Fluor™) Antibody, CD4, RM4-4, BioLegend, 285548

RM4-4 antibody does not block the binding of GK1.5 and RM4-5 antibodies to CD4 T cells. For immunohistochemistry applications, the RM4-5 (Cat. No. 100506) and GK1.5 (Cat. No. 100402) antibodies are recommended.

Spark YG™ 581 anti-mouse CD4 (Flexi-Fluor™) Antibody, CD4, RM4-5, BioLegend, 285409

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding,in vivodepletion of CD4+cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.100575 and 100576).

Spark YG™ 581 anti-human CD8a (Flexi-Fluor™) Antibody, CD8a, RPA-T8, BioLegend, 285367

The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).

Spark YG™ 581 anti-mouse/rat XCR1 (Flexi-Fluor™) Antibody, XCR1, ZET, BioLegend, 285418

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues2.

Spark YG™ 581 anti-human CD117 (c-kit) (Flexi-Fluor™) Antibody, CD117, 104D2, BioLegend, 285540

The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.

Spark YG™ 581 anti-mouse CD170 (Siglec-F) (Flexi-Fluor™) Antibody, CD170, S17007L, BioLegend, 285537

Spark YG™ 581 anti-mouse F4/80 (Flexi-Fluor™) Antibody , F4/80, W20065D, BioLegend, 285415

Spark YG™ 581 anti-human CD56 (NCAM) Recombinant Antibody (Flexi-Fluor™), CD56, QA17A16, BioLegend, 285396

Spark YG™ 581 anti-human CD206 (MMR) (Flexi-Fluor™) Antibody, CD206, 15-2, BioLegend, 285541

The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting1, blocking of ligand binding1,2, immunofluorescence3, and immunohistochemical staining of acetone-fixed frozen tissue sections1. The Utra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321149 and 321150).