Search results for 581

Spark YG™ 581 anti-mouse CD107a (LAMP-1) (Flexi-Fluor™) Antibody, CD107a, 1D4B, BioLegend, 285525

Spark YG™ 581 anti-human CD154 (CD40L) (Flexi-Fluor™) Antibody, CD154, 24-31, BioLegend, 285526

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1,3and blocking of T cell-dependent B cell differentiation1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Spark YG™ 581 anti-mouse CD117 (c-kit) (Flexi-Fluor™) Antibody, CD117, 2B8, BioLegend, 285542

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Spark YG™ 581 anti-human CD56 (NCAM) (Flexi-Fluor™) Antibody, CD56 (NCAM), 5.1H11, BioLegend, 285379

Spark YG™ 581 anti-mouse CD127 (IL-7Rα) (Flexi-Fluor™) Antibody, CD127, A7R34, BioLegend, 285519

Clone A7R34 is able to completely block the binding of clone S18006K to IL-7Rα. It does not block SB/199.

Spark YG™ 581 anti-human CD279 (PD-1) (Flexi-Fluor™) Antibody, CD279, EH12.2H7, BioLegend, 285389

Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections13, and spatial biology (IBEX)15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Spark YG™ 581 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) Antibody, CD326, G8.8, BioLegend, 285390

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Spark YG™ 581 anti-human CD107a (LAMP-1) (Flexi-Fluor™) Antibody, CD107a, H4A3, BioLegend, 285394

Additional reported applications (for the relevant formats) include: Western blotting8, immunohistochemical staining2, immunofluorescence5,7, and immunoprecipitation5.This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Spark YG™ 581 anti-mouse Ly-6C (Flexi-Fluor™) Antibody, Ly-6C, HK1.4, BioLegend, 285400

Clone HK1.4 does not block the binding of clone RB6-8C58.Additional reported applications (for relevant formats of this clone) include:in vitroactivation of T cells1-3and immunohistochemistry of frozen sections4.

Spark YG™ 581 anti-human HLA-DR (Flexi-Fluor™) Antibody, HLA-DR, L243, BioLegend, 285403

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8,in vitroblocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).