Search results for APC/Fire
APC/Fire™ 810 anti-human CD14 Antibody, CD14, M5E2, BioLegend, 301869
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
APC/Fire™ 810 anti-human CD14 Antibody, CD14, M5E2, BioLegend, 301870
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
APC/Fire 750 anti-human CD14 Antibody anti-CD14 - M5E2, BioLegend, 301853
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
APC/Fire 750 anti-human CD14 Antibody anti-CD14 - M5E2, BioLegend, 301854
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
APC/Fire 750 anti-human CD271 NGFR Antibody, CD271, ME20.4, BioLegend, 345115
Additional reported applications for the relevant formats include: immunoprecipitation1, Western blotting4, immunohistochemical staining of frozen or paraffin embedded tissue sections1,2, and blocking of NGF binding1.
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
APC/Fire 750 anti-human CD271 NGFR Antibody, CD271, ME20.4, BioLegend, 345116
Additional reported applications for the relevant formats include: immunoprecipitation1, Western blotting4, immunohistochemical staining of frozen or paraffin embedded tissue sections1,2, and blocking of NGF binding1.
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
APC/Fire 750 anti-mouse CD31 Antibody anti-CD31 - MEC133, BioLegend, 102527
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8Additional reported applications (in the relevant formats) include: immunoprecipitation1,in vitroandin vivoblocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.Special Note:The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.102529 and 102530).
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
APC/Fire 750 anti-mouse CD31 Antibody anti-CD31 - MEC133, BioLegend, 102528
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8Additional reported applications (in the relevant formats) include: immunoprecipitation1,in vitroandin vivoblocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.Special Note:The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.102529 and 102530).
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
APC/Fire™ 810 anti-mouse CD11c Antibody, CD11c, N418, BioLegend, 117383
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9(Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
APC/Fire™ 810 anti-mouse CD11c Antibody, CD11c, N418, BioLegend, 117384
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9(Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
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