Search results for 718

Spark Red™ 718 anti-human CD314 (NKG2D) (Flexi-Fluor™) Antibody, CD314, 1D11, BioLegend, 285204

The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.

Spark Red™ 718 anti-human CD154 (Flexi-Fluor™) Antibody, CD154, 24-31, BioLegend, 285037

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1,3and blocking of T cell-dependent B cell differentiation1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Spark Red™ 718 anti-human CX3CR1 (Flexi-Fluor™) Antibody, CX3CR1, 2A9-1, BioLegend, 285040

Spark Red™ 718 anti-human CD99 (Flexi-Fluor™) Antibody, CD99, 3B2/TA8, BioLegend, 285046

Spark Red™ 718 anti-human CD36 (Flexi-Fluor™) Antibody, CD36, 5-271, BioLegend, 285049

Additional reported applications (for the relevant formats) include: immunofluorescence2.

Spark Red™ 718 anti-mouse CD8a (Flexi-Fluor™) Antibody, CD8a, 53-6.7, BioLegend, 285050

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Spark Red™ 718 anti-human CD90 (Thy1) (Flexi-Fluor™) Antibody, CD90, 5E10, BioLegend, 285208

Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.

Spark Red™ 718 anti-human CD193 (CCR3) (Flexi-Fluor™) Antibody, CD193, 5E8, BioLegend, 285027

Additional reported applications (for the relevant formats) include: The 5E8 antibody is useful for immunofluorescent staining and flow cytometric analysis of CCR3 expression.It has been observed that the 5E8 antibody clone can interact with PE/Cyanine7 antibody conjugates during multi-color staining, potentially leading to unwanted staining. This interaction can be resolved by sequentially staining with the 5E8 antibody first and then followed by the PE/Cyanine7 conjugate of interest.

Spark Red™ 718 anti-human CD335 (NKp46) (Flexi-Fluor™) Antibody, CD335, 9E2, BioLegend, 285024

Clone 9E2 has been shown to block NK activation through NKp46.6

Spark Red™ 718 anti-mouse CD45RB (Flexi-Fluor™) Antibody, CD45RB, C363-16A, BioLegend, 285352

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.