Search results for B7-H1
Brilliant Violet 785 anti-human CD274 B7-H1, PD-L1 Antibody anti-CD274 - 29E.2A3, BioLegend, 329735
Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748).It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.Clone 29E.2A3 does not work in Western blot applications7.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Brilliant Violet 785 anti-human CD274 B7-H1, PD-L1 Antibody anti-CD274 - 29E.2A3, BioLegend, 329736
Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748).It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.Clone 29E.2A3 does not work in Western blot applications7.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark YG™ 570 anti-human CD274 (B7-H1, PD-L1) Antibody, CD274, 29E.2A3, BioLegend, 329760
Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748).It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.Clone 29E.2A3 does not work in Western blot applications7.
Host
Mouse
Reactivity
Human
Applications
IHC-P - Quality testedFC - Verified
Conjugation
Brilliant Violet 510 anti-human CD274 B7-H1, PD-L1 Antibody anti-CD274 - 29E.2A3, BioLegend, 329733
Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748).It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.Clone 29E.2A3 does not work in Western blot applications7.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Brilliant Violet 510 anti-human CD274 B7-H1, PD-L1 Antibody anti-CD274 - 29E.2A3, BioLegend, 329734
Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748).It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.Clone 29E.2A3 does not work in Western blot applications7.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Ultra-LEAF™ Purified anti-mouse CD274 (B7-H1, PD-L1) - Antibody anti-CD274 - MIH6, BioLegend, 153602
mAb MIH6 blocks the binding of mouse PD-L1 to PD-1 (CD279)
Host
Rat
Reactivity
Mouse
Applications
FC -Quality testedBlocking -Reported in the literature, not verified in house
Conjugation
Ultra-LEAF™ Purified anti-mouse CD274 (B7-H1, PD-L1) - Antibody anti-CD274 - MIH6, BioLegend, 153603
mAb MIH6 blocks the binding of mouse PD-L1 to PD-1 (CD279)
Host
Rat
Reactivity
Mouse
Applications
FC -Quality testedBlocking -Reported in the literature, not verified in house
Conjugation
PE/Dazzle 594 anti-mouse CD274 B7-H1, PD-L1 Antibody anti-CD274 - 10F.9G2, BioLegend, 124323
Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
PE/Dazzle 594 anti-mouse CD274 B7-H1, PD-L1 Antibody anti-CD274 - 10F.9G2, BioLegend, 124324
Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
PE/Fire™ 640 anti-mouse CD274 (B7-H1, PD-L1) Antibody, CD274, 10F.9G2, BioLegend, 124345
Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
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