Search results for Ki-67 antibody (Ki-67)

Spark PLUS UV395™ anti-human Ki-67 Antibody, Ki-67, Ki-67, BioLegend, 350544

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.

Brilliant Violet 650™ anti-human Ki-67 Antibody, Ki-67, Ki-67, BioLegend, 350537

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.

Brilliant Violet 650™ anti-human Ki-67 Antibody, Ki-67, Ki-67, BioLegend, 350538

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.

Brilliant Violet 785™ anti-human Ki-67 Antibody, Ki-67, Ki-67, BioLegend, 350539

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.

Brilliant Violet 785™ anti-human Ki-67 Antibody, Ki-67, Ki-67, BioLegend, 350540

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.

Brilliant Violet 750™ anti-human Ki-67 Antibody, Ki-67, Ki-67, BioLegend, 350535

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.

Brilliant Violet 750™ anti-human Ki-67 Antibody, Ki-67, Ki-67, BioLegend, 350536

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.

PE/Dazzle™ 594 anti-mouse/human Ki-67 Antibody, Ki-67, 11F6, BioLegend, 151219

PE/Dazzle™ 594 anti-mouse/human Ki-67 Antibody, Ki-67, 11F6, BioLegend, 151220

Brilliant Violet 785™ anti-mouse/human Ki-67 Antibody, Ki-67, 11F6, BioLegend, 151229