Search results for TWEAK antibody
Purified anti-human CD255 TWEAK Antibody anti-CD255 - CARL-1, BioLegend, 308302
Flow Cytometry:For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 308304) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).ELISA Detection:The biotinylated CARL-1 antibody is useful as the detection antibody in sandwich ELISA for measuring human TWEAK protein, in combination with CARL-2 antibody (Cat. No. 308402) used as ELISA capture.Neutralization1,2:In a dose-dependent manner, CARL-1 is more effective than CARL-21. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 308307).
Host
Mouse
Reactivity
Human
Applications
FC - Quality testedIHC - Reported in the literature, not verified in house
Conjugation
PE anti-human CD255 TWEAK Antibody anti-CD255 - CARL-1, BioLegend, 308305
Flow Cytometry:For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 308304) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).ELISA Detection:The biotinylated CARL-1 antibody is useful as the detection antibody in sandwich ELISA for measuring human TWEAK protein, in combination with CARL-2 antibody (Cat. No. 308402) used as ELISA capture.Neutralization1,2:In a dose-dependent manner, CARL-1 is more effective than CARL-21. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 308307).
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Ultra-LEAF™ Purified anti-mouse CD255 (TWEAK) Antibody, CD255, MTW-1, BioLegend, 120009
Additional reported applications (for the relevant formats) include:in vitroblocking of ligand binding2. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 120009 & 120010).
Host
Rat
Reactivity
Mouse
Applications
FC -Quality testedBlock -Reported in the literature, not verified in house
Conjugation
Ultra-LEAF™ Purified anti-mouse CD255 (TWEAK) Antibody, CD255, MTW-1, BioLegend, 120010
Additional reported applications (for the relevant formats) include:in vitroblocking of ligand binding2. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 120009 & 120010).
Host
Rat
Reactivity
Mouse
Applications
FC -Quality testedBlock -Reported in the literature, not verified in house
Conjugation
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