Search results for 718
Spark Red™ 718 anti-human HLA-DR (Flexi-Fluor™) Antibody, HLA-DR, L243, BioLegend, 285085
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8,in vitroblocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC
Conjugation
Spark Red™ 718 anti-mouse/human CD11b (Flexi-Fluor™) Antibody, CD11b, M1/70, BioLegend, 285219
Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4,in vitroblocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).
Host
Rat
Reactivity
Mouse, Human
Applications
FC
Conjugation
Spark Red™ 718 anti-mouse I-A/I-E Antibody, I-A/I-E, M5/114.15.2, BioLegend, 107675
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-EkMHC class II alloantigens from mice carrying H-2p,r,q,b,d,uhaplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-AsMHC class II alloantigens.1Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6,in vitroandin vivoblocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-mouse I-A/I-E Antibody, I-A/I-E, M5/114.15.2, BioLegend, 107676
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-EkMHC class II alloantigens from mice carrying H-2p,r,q,b,d,uhaplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-AsMHC class II alloantigens.1Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6,in vitroandin vivoblocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-human CD138 (Syndecan-1) (Flexi-Fluor™) Antibody, CD138, MI15, BioLegend, 285093
The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns onin vitrogenerated plasma cells and some CD138+cell lines.4Additional reported applications for the relevant formats include: immunofluorescent staining1, Western blotting2, immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections3, and spatial biology (IBEX)5,6.
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation
Spark Red™ 718 anti-human CD279 (PD-1) (Flexi-Fluor™) Antibody, CD279, NAT105, BioLegend, 285096
Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.We recommend using our Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) (Cat. No. 929501).
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation
Spark Red™ 718 anti-human CD49e (Flexi-Fluor™) Antibody, CD49e, NKI-SAM-1, BioLegend, 285362
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and inhibition of a5 integrin mediated cell adhesion2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 328004).
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation
Spark Red™ 718 anti-human CD337 (NKp30) (Flexi-Fluor™) Antibody, CD337, P30-15, BioLegend, 285098
Additional reported applications (for the relevant formats) include: ELISA, stimulation of human NK cells via NKp30 in a redirected lysis assay, and blocking of NKp30 function in solution1,3,5. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 325223 & 325224).
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation
Spark Red™ 718 anti-human CD336 (NKp44) (Flexi-Fluor™) Antibody, CD336, P44-8, BioLegend, 285099
The p44-8 antibody against human NKp44 has been shown to be useful for flow cytometry, stimulation of human NK cells via NKp44 in a redirected lysis assay, and blocking of NKp44 function in solution. Additional reported applications (for the relevant formats) include: stimulation of human NK cells via NKp44 in a redirected lysis assay, and blocking of NKp44 function in solution1,2. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 325104).
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation
Spark Red™ 718 anti-mouse CD172a (SIRPα) (Flexi-Fluor™) Antibody, CD172a (SIRPα), P84, BioLegend, 285101
Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD474,in vivoblocking of dendritic cell migration3, enhancing of macrophage phagocytosis2,4, immunohistochemical staining of cerebellum frozen sections1, immunoprecipitation2,4, and spatial biology (IBEX)5,6.
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation
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