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Western Blotting: 1:1000 Fluorescent Western: 1:1000 Simple Western^TM: 1:50 - 1:250 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:1600

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Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200

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Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200

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Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Frozen): 1:400 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:1000

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Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:250 - 1:1000

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Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Flow Cytometry (Live): 1:200 - 1:800

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Western Blotting: 1:1000 Simple Western^TM: 1:10 - 1:50 Immunoprecipitation: 1:100 IHC Leica Bond: 1:50 Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200

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Source / PurificationMonoclonal antibody is produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr1068 of human EGFR

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(1)

or to Tyr1289 of human HER3/ErbB3. Monoclonal antibody to EGFR is produced by immunizing animals with a synthetic GST-fusion protein corresponding to residues containing the cytoplasmic domain of human EGF receptor.

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(1)

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(#5S21)

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Isotype

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IgG-like

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[(VK-CK-VH-CH1-h-CH2-CH3_L-kappa)_Fab-G1-kappa]-dimer

(1)

Search results for EGFR

Showing 1 – 10 of729products

ID: BAB939773274

PE anti-EGFR Phospho (Tyr845) Recombinant Antibody, EGFR, QA19A93, BioLegend, 943404

This clone was tested for ICC on A-431 cells treated with or without EGF. We failed to detect any EGFR Phospho (Tyr845) staining using the following fixation and permeabilization methods:4% PFA plus Triton X-1004% PFA plus methanolMethanol onlyThis clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells.This recombinant antibody clone is derived from clone A18028A.This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and mouse (Tyr847) orthologs due to complete sequence conservation with the immunizing peptide.

Host

Mouse

Reactivity

Human

Applications

ICFC - Quality tested

Conjugation

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ID: BAB676854478

Alexa Fluor® 647 anti-EGFR Antibody, EGFR, A19002A, BioLegend, 933903

This clone was tested for ICC using 4% PFA-fixed A431 cells permabilized with methanol or Triton X-100. While both permeabilization methods were compatible with the antibody, methanol enabled superior staining.This clone is predicted to recognize isoform I based off of complete sequence homology of the immunizing peptide and the corresponding region of the isoform.

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