Search results for 421

Brilliant Violet 421 anti-human IgM Antibody anti-IgM - MHM-88, BioLegend, 314515
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation

Brilliant Violet 421 anti-human IgM Antibody anti-IgM - MHM-88, BioLegend, 314516
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation

Brilliant Violet 421™ anti-mouse CD20 Recombinant Antibody, CD20, QA18A73, BioLegend, 161411
This recombinant antibody clone is derived from clone SA271G2.
Host
Mouse
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation

Brilliant Violet 421 anti-human CD184 CXCR4 Antibody anti-CD184 - 12G5, BioLegend, 306517
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections11, immunocytochemistry3, immunofluorescence microscopy2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-14,5. Clone 12G5 may not be suitable for Western blotting.10The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation

Brilliant Violet 421 anti-human CD184 CXCR4 Antibody anti-CD184 - 12G5, BioLegend, 306518
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections11, immunocytochemistry3, immunofluorescence microscopy2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-14,5. Clone 12G5 may not be suitable for Western blotting.10The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation

Brilliant Violet 421 anti-mouse CD3epsilon Antibody anti-CD3e - 145-2C11, BioLegend, 100335
Clone 145-2C11 is useful forin vitroblocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32, andin vivoT cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6,in vitroandin vivostimulation of T cells1,2,7,12,16, immunofluorescent staining5, andin vivoT cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC -Quality testedICC -Verified
Conjugation

Brilliant Violet 421 anti-mouse CD3epsilon Antibody anti-CD3e - 145-2C11, BioLegend, 100341
Clone 145-2C11 is useful forin vitroblocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32, andin vivoT cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6,in vitroandin vivostimulation of T cells1,2,7,12,16, immunofluorescent staining5, andin vivoT cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC -Quality testedICC -Verified
Conjugation

Brilliant Violet 421 anti-mouse CD3epsilon Antibody anti-CD3e - 145-2C11, BioLegend, 100336
Clone 145-2C11 is useful forin vitroblocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32, andin vivoT cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6,in vitroandin vivostimulation of T cells1,2,7,12,16, immunofluorescent staining5, andin vivoT cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC -Quality testedICC -Verified
Conjugation

Brilliant Violet 421 anti-mouse CD80 Antibody anti-CD80 - 16-10A1, BioLegend, 104725
Additional reported applications (for the relevant formats) include: immunoprecipitation2,in vitroandin vivoblocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC - Quality testedIHC-F - Verified
Conjugation

Brilliant Violet 421 anti-mouse CD80 Antibody anti-CD80 - 16-10A1, BioLegend, 104726
Additional reported applications (for the relevant formats) include: immunoprecipitation2,in vitroandin vivoblocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC - Quality testedIHC-F - Verified
Conjugation
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