Search results for 650

Brilliant Violet 650™ anti-human CD135 (Flt-3/Flk-2) Antibody, CD135, BV10A4H2, BioLegend, 313330

Brilliant Violet 650™ anti-mouse TCR γ/δ Antibody, TCR γ/δ, GL3, BioLegend, 118147

The GL3 antibody has been shown to be useful in identifying γ/δ T cells by flow cytometry and immunohistochemistry and depleting γ/δ T cellsin vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, andin vivodepletion of γ/δ T cells3-5.

Brilliant Violet 650™ anti-human TCR α/β Antibody, TCR α/β, IP26, BioLegend, 306753

Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone. Other anti-CD3 clones may compete out the binding of IP26.

Brilliant Violet 650™ anti-human TCR α/β Antibody, TCR α/β, IP26, BioLegend, 306754

Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone. Other anti-CD3 clones may compete out the binding of IP26.

Brilliant Violet 650™ anti-human CD273 (B7-DC, PD-L2) Antibody, CD273, MIH18, BioLegend, 345529

Additional reported applications (for the relevant formats) include: blocking4,5, and immunohistochemistry in frozen sections2and paraffin-embedded formalin-fixed sections6. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 345504).

Brilliant Violet 650™ anti-human CD273 (B7-DC, PD-L2) Antibody, CD273, MIH18, BioLegend, 345530

Additional reported applications (for the relevant formats) include: blocking4,5, and immunohistochemistry in frozen sections2and paraffin-embedded formalin-fixed sections6. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 345504).

Brilliant Violet 650 anti-mouse CD274 B7-H1, PD-L1 Antibody anti-CD274 - 10F.9G2, BioLegend, 124336

Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Brilliant Violet 650 anti-human CD274 B7-H1, PD-L1 Antibody anti-CD274 - 29E.2A3, BioLegend, 329739

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748).It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.Clone 29E.2A3 does not work in Western blot applications7.

Brilliant Violet 650 anti-human CD274 B7-H1, PD-L1 Antibody anti-CD274 - 29E.2A3, BioLegend, 329740

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748).It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.Clone 29E.2A3 does not work in Western blot applications7.

Brilliant Violet 650™ anti-mouse TCR β chain Antibody, TCR β chain, H57-597, BioLegend, 109251

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2,in vitrostimulation2,3,in vivodepletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).