Search results for 718

Spark Red™ 718 anti-mouse TCR γ/δ (Flexi-Fluor™) Antibody, TCR γ/δ, GL3, BioLegend, 285072

The GL3 antibody has been shown to be useful in identifying γ/δ T cells by flow cytometry and immunohistochemistry and depleting γ/δ T cellsin vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, andin vivodepletion of γ/δ T cells3-5.

Spark Red™ 718 anti-human TCR α/β (Flexi-Fluor™) Antibody, TCR α/β, IP26, BioLegend, 285079

Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone. Other anti-CD3 clones may compete out the binding of IP26.

Spark Red™ 718 anti-human CD223 (LAG-3) (Flexi-Fluor™) Antibody, CD223 (LAG-3), 11C3C65, BioLegend, 285029

The staining of clone 11C3C65 cannot be blocked by clone 7H2C65, which is another anti-human CD223 (LAG-3) antibody.

Spark Red™ 718 anti-human HLA-A,B,C (Flexi-Fluor™) Antibody, HLA-A,B,C, W6/32, BioLegend, 285117

Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Spark Red™ 718 anti-mouse TCR Vβ5.1 5.2 (Flexi-Fluor™) Antibody, TCR Vβ5.1, 5.2, MR9-4, BioLegend, 285347

Additional reported applications (for the relevant formats) include: Induction of proliferation of Vß5.1+and Vß5.2+T cells2, 3andin vivodepletion of Vß5+T cells4.

Spark Red™ 718 anti-human CD66a/c/e (Flexi-Fluor™) Antibody, CD66a/c/e, ASL-32, BioLegend, 285195

Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™) Antibody, TCR β chain, H57-597, BioLegend, 285073

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2,in vitrostimulation2,3,in vivodepletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Spark Red™ 718 anti-mouse TCR Vβ8.1 8.2 (Flexi-Fluor™) Antibody, TCR Vβ8.1, 8.2, KJ16-133.18, BioLegend, 285353

Spark Red™ 718 anti-rat CD90/mouse CD90.1 (Thy1.1) (Flexi-Fluor™) Antibody, CD90/CD90.1, OX-7, BioLegend, 285340

The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.Additional reported applications (for the relevant formats) include: immunohistochemical7and immunofluorescent8staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation1, Western blotting1,in vitroactivation of leukocytes2, induction of endothelial cell permeability3, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritisin vivo4.

Spark Red™ 718 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™) Antibody, TER-119, TER-119, BioLegend, 285222

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).