Search results for 718

Spark Red™ 718 anti-human CD172a/b (SIRPα/β) (Flexi-Fluor™) Antibody, CD172a/b (SIRPα/β), SE5A5, BioLegend, 285111

Clone SE5A5 recognizes a common epitope on SIRPa (90 kD) and SIRPß (50 kD)3. A high degree of homology has been found between SIRP family isoforms alpha and beta at the level of extracellular domains. Consequently, many anti SIRP antibody clones, such as SE5A5, have been reported to cross react with several SIRP isoforms1,4,5. It reacts with CD172a and has weak cross-reaction with CD172b. This antibody is able to block the binding of SIRPa (SIRPa1 and SIRPa2) to CD471,6.

Spark Red™ 718 anti-human HLA-DR, DP, DQ (Flexi-Fluor™) Antibody, HLA-DR, DP, DQ, Tü39, BioLegend, 285223

Tü39 has been reported to react with a shared epitope of HLA-DR, HLA-DP, and HLA-DQ.Additional reported applications (of relevant formats) include immunoprecipitation6,in vitroblocking of MLR5, and suppressor cell generation4. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).

Spark Red™ 718 anti-mouse Ly-6A/E (Sca-1) (Flexi-Fluor™) Antibody, Ly-6A/E, E13-161.7, BioLegend, 285200

Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.The two Sca-1 recognizing clonesD7and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7

Spark Red™ 718 anti-human Ig light chain κ (Flexi-Fluor™) Antibody, Ig light chain κ, MHK-49, BioLegend, 285090

Spark Red™ 718 anti-human Ig light chain λ (Flexi-Fluor™) Antibody, Ig light chain λ, MHL-38, BioLegend, 285091

Clone MHL-38 is not compatible with Human TruStain FcX (Fc Receptor Blocking Solution) (Cat. No. 422301 & 422302).

Spark Red™ 718 anti-mouse Ly-6G/Ly-6C (Gr-1) (Flexi-Fluor™) Antibody, Ly-6G/Ly-6C, RB6-8C5, BioLegend, 285221

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytesin vivo. Additional reported applications (for relevant formats of this clone) include:in vitrocomplement-mediated cytotoxicity2,in vivodepletion3-5,9, immunoprecipitation1, immunohistochemical staining6(including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.Special Note:Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).