Search results for 785
Brilliant Violet 785™ anti-human CD137 (4-1BB) Antibody, CD137, 4B4-1, BioLegend, 309850
Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Brilliant Violet 785 anti-mouse/human KLRG1 MAFA Antibody anti-KLRG1 - 2F1/KLRG1, BioLegend, 138429
For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421™, or APC, are recommended.Additional reported applications (for relevant formats) include: Western Blotting1.
Host
Syrian Hamster
Reactivity
Mouse, Human
Applications
FC - Quality tested
Conjugation
Brilliant Violet 785 anti-human TCR Valpha7.2 Antibody anti-TCR V alpha7.2 - 3C10, BioLegend, 351721
Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Brilliant Violet 785 anti-human TCR Valpha7.2 Antibody anti-TCR V alpha7.2 - 3C10, BioLegend, 351722
Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Brilliant Violet 785 anti-mouse CD366 Tim-3 Antibody anti-CD366 - RMT3-23, BioLegend, 119725
Additional reported applications (for relevant formats) include:in vitro1andin vivo2blocking of Tim-3, and immunohistochemical staining of frozen sections2. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 119731-119736).
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
Brilliant Violet 785™ anti-mouse CD127 (IL-7Rα) Antibody, CD127, SB/199, BioLegend, 121131
Additional reported applications (for the relevant formats) include: To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Brilliant Violet 785 anti-mouse IL-17A Antibody anti-IL-17A - TC11-18H10.1, BioLegend, 506928
ELISA Capture3,4and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.Neutralization6,9:The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivityin vivoandin vitro(Cat. No. 506906).
Host
Rat
Reactivity
Mouse
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 785™ anti-human IL-4 Antibody, IL-4, MP4-25D2, BioLegend, 500845
ELISA Capture or ELISpot Capture: The MP4-25D2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay.Flow Cytometry6,9:The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.Neutralization1-3:The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.
Host
Rat
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 785™ anti-human IL-4 Antibody, IL-4, MP4-25D2, BioLegend, 500846
ELISA Capture or ELISpot Capture: The MP4-25D2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay.Flow Cytometry6,9:The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.Neutralization1-3:The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.
Host
Rat
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 785 anti-mouse TNF-alpha Antibody anti-TNF-alpha - MP6-XT22, BioLegend, 506341
ELISA Capture: The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF-α (Cat. No. 575209) as the standard.Flow Cytometry6,11,12:The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.Neutralization1,5,10,16,17:The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF-α. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse TNF-α bioactivityin vivoandin vitro(Cat. No. 506310). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections7-9in vivodetection5, immunofluorescence, and immunocytochemistry.Note: For testing mouse TNF-α in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430901) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ICFC - Quality tested
Conjugation
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