Search results for 785
Brilliant Violet 785™ anti-mouse IL-2 Antibody, IL-2, JES6-5H4, BioLegend, 503843
ELISA Detection1-3or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 & 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard.Flow Cytometry8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations.Neutralization1,7: The Ultra-LEAF™ purified antibody (Endotoxinin vivoandin vitro(Cat. No. 503845-503850)) is recommended for neutralization.Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections2,in vivocapture7, and immunocytochemistry.Note:For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. No. 431001 & 431004) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ICFC -Quality tested
Conjugation
Brilliant Violet 785™ anti-human Ki-67 Antibody, Ki-67, Ki-67, BioLegend, 350539
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 785™ anti-human Ki-67 Antibody, Ki-67, Ki-67, BioLegend, 350540
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 785 anti-human/mouse/rat CD278 ICOS Antibody anti-CD278 - C398.4A, BioLegend, 313533
The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitrocostimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).
Host
Armenian Hamster
Reactivity
Human, Mouse, Rat
Applications
FC - Quality tested
Conjugation
Brilliant Violet 785 anti-human/mouse/rat CD278 ICOS Antibody anti-CD278 - C398.4A, BioLegend, 313534
The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitrocostimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).
Host
Armenian Hamster
Reactivity
Human, Mouse, Rat
Applications
FC - Quality tested
Conjugation
Brilliant Violet 785 anti-human CD366 Tim-3 Antibody anti-CD366 - F38-2E2, BioLegend, 345031
Additional reported applications (for relevant formats of this clone) include: costimulation1(clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Brilliant Violet 785 anti-human CD366 Tim-3 Antibody anti-CD366 - F38-2E2, BioLegend, 345032
Additional reported applications (for relevant formats of this clone) include: costimulation1(clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Brilliant Violet 785™ anti-human/mouse integrin β7 Antibody, Integrin β7, FIB504, BioLegend, 321243
The FIB504 antibody has been reported to react with mouse and human ß7 integrin and to block ß7 integrin-mediated cell adhesion inin vitroandin vivostudies. Additional reported applications (for the relevant formats) include: blocking of cell adhesion1,3,4. The Ultra-LEAF™ purified FIB504 antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321233-321238).
Host
Rat
Reactivity
Human, Mouse, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
Brilliant Violet 785™ anti-human/mouse integrin β7 Antibody, Integrin β7, FIB504, BioLegend, 321244
The FIB504 antibody has been reported to react with mouse and human ß7 integrin and to block ß7 integrin-mediated cell adhesion inin vitroandin vivostudies. Additional reported applications (for the relevant formats) include: blocking of cell adhesion1,3,4. The Ultra-LEAF™ purified FIB504 antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321233-321238).
Host
Rat
Reactivity
Human, Mouse, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
Brilliant Violet 785™ anti-mouse CD64 (FcγRI) Antibody, CD64, X54-5/7.1, BioLegend, 139340
The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
Host
Mouse
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
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