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Suggested for Flow Cytometry

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not verified in house

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FC. Capture Ab

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Functional assay

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Immunohistochemistry (Paraffin): 1:50 - 1:200

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Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200

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PG - Quality tested

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Research in vivo

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Single Cell (Intra)

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WB ICC/IF IHC-P IP ELISA PLA

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Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Live): 1:400

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Western Blotting: 1:1000 Immunoprecipitation: 1:50 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:50 - 1:100 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800

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Phycoerythrin-Cyanine7 (PE-Cy7) Wavelength

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APC Fluorescent Dye

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APC. Ex:650nm. Em:660nm.

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FITC

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PE-DyLight594

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PE. Ex:565nm. Em:574nm.

(2)

Peridinin-chlorophyll proteins (PerCP) Wavelength

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5CFLX

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ABflo® 450. Ex:406nm. Em:445nm.

(1)

ABflo® 488. Ex:491nm. Em:516nm.

(1)

ABflo® 500. Ex:410nm. Em:501nm.

(1)

ABflo® 610. Ex:421nm. Em:612nm.

(1)

APC-Cy7. Ex:651nm. Em:779nm.

(1)

Biotin The reagent is free of unconjugated biotin.

(1)

PE-Cy7. Ex:565nm. Em:778nm.

(1)

PerCP-Cy5.5. Ex:482nm. Em:695nm.

(1)

Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5) Wavelength

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Phycoerythrin-Cyanine5 (PE-Cy5) Wavelength

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Recombinant

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Clone Number

5.1H11

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QA17A16

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HCD56

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MEM-188

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QA18A21

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W22097A

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ARC71418

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LT56

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N901-DM1

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AMC0687

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H796

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SAA2213

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U262

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A19063A

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N901

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39D5

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B-A19

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YA3780

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(#5F27)

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ARC60182

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B159.52

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ERIC-1

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SAA2007

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W22098A

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W22098E

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YA3222

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YA4690

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YA5643

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Isotype

Mouse IgG1, κ

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IgG

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IgG2a

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IgG1

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Mouse IgG2a, κ

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Rat IgG2a, κ

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Mouse IgG2b, κ

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IgG2a Kappa

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Rabbit IgG.

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IgG2

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Mouse IgG1

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Mouse IgG2a

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lgG1

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IgG1 kappa

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